Stability of bioactive solid lipid nanoparticles loaded with herbal extracts when exposed to simulated gastrointestinal tract conditions. (December 2015)

• Record Type: Journal Article
• Authors: Campos, Débora A.; Madureira, Ana Raquel; Sarmento, Bruno; Gomes, Ana Maria; Pintado, Maria Manuela
• Is Part Of: Food research international. Volume 78(2015:Dec.)
• Page Start: 131
• Page End: 140
• Publication Date: 2015-12
• Subjects: SLN -- Herbal extracts -- Digestion -- Antioxidant activity -- Release profiles; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2015.10.025
• Languages: English
• ISSNs: 0963-9969

Abstract

ABSTRACT: Solid lipid nanoparticles (SLN) can be used as vehicles for phenolic compounds rich extracts. In the present work two types of waxes — witepsol and carnauba were tested for the first time in the production of solid lipid nanoparticles (WSLN and CSLN, respectively) loaded with sage and savoury extracts. Physical characterization and association efficiencies calculation were performed. Discrimination of loaded phenolic compounds from each extract was made using HPLC assays. Antioxidant activities of SLN were characterized using two different methods — ABTS and ORAC. Finally, the phenolic compound release profile from SLN and stability when exposed to simulated gastrointestinal tract (GIT) conditions were also evaluated. Different phenolic compounds from sage and savoury extracts were entrapped in SLN. The highest antioxidant activity was obtained for the SLN loaded with savoury extract. Stomach simulated condition provokes a partial release of rosmarinic acid from SLN, whereas at small intestine simulation step, all SLN showed a release of ca. 100%. Witepsol SLN were the ones that best maintained their physical integrity during digestion, showing to be the most stable vehicles for sage and savoury extracts. These SLN show to be suitable for the production of food functional ingredients bearing antioxidant activity. Highlights: Phenolic compounds from sage and savoury extracts are entrapped in SLN. SLN loaded with savoury extracts showed higher antioxidant activity. The digestion of SLN is affected by the type of wax used at production. At the small intestine all SLNs showed ca. 100% release of rosmarinic acid. Witepsol SLNs showed to be the most stable vehicles for sage and savoury extracts.