Coffee silverskin extract improves glucose-stimulated insulin secretion and protects against streptozotocin-induced damage in pancreatic INS-1E beta cells. (November 2016)

• Record Type: Journal Article
• Authors: Fernandez-Gomez, Beatriz; Ramos, Sonia; Goya, Luis; Mesa, María Dolores; del Castillo, María Dolores; Martín, María Ángeles
• Is Part Of: Food research international. Volume 89(2016) Part 2
• Page Start: 1015
• Page End: 1022
• Publication Date: 2016-11
• Subjects: CF caffeine -- CGA chlorogenic acid -- CSE coffee silverskin extract -- DCF dichlorofluorescein -- GPx glutathione peroxidase -- GR glutathione reductase -- GSH reduced glutathione -- GSIS glucose-stimulated insulin secretion -- KRB Krebs–Ringer bicarbonate buffer -- OPT o-phthaldehyde -- ROS reactive oxygen species -- STZ streptozotocin -- t-BOOH tert-butylhydroperoxide -- T2D Type 2 diabetes; Coffee silverskin -- Coffee by-products -- Oxidative stress -- Insulin secretion -- Antidiabetic effect; Food -- Analysis -- Periodicals; Food industry and trade -- Periodicals; Food industry and trade -- Canada -- Periodicals; Food Technology -- Periodicals; Food -- Periodicals; Food-Processing Industry -- Periodicals; Aliments -- Industrie et commerce -- Périodiques; Aliments -- Industrie et commerce -- Canada -- Périodiques; Aliments -- Recherche -- Périodiques; Food industry and trade; Canada; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.foodres.2016.03.006
• Languages: English
• ISSNs: 0963-9969

Abstract

Abstract: The present research aimed to provide novel information regarding the antidiabetic mechanism of action of coffee silverskin extract (CSE) and its components chlorogenic acid (CGA) and caffeine (CF). Their effect on insulin secretion and biomarkers of oxidative stress in INS-1E cells in vitro cultured under physiological and stressed conditions were assessed. Under physiological conditions, CSE and pure CGA and CF did not affect cells´ oxidative status and viability. However, concentrations of CSE ≥ 1 μg/mL and CGA ≥ 5 μM significantly increased (p < 0.05) the enzymatic activity of glutathione peroxidase (GPx). Moreover, all concentrations of CSE (1–10 μg/mL) and the dose of 10 μM of CGA, significantly stimulated (p < 0.05) insulin secretion in cells cultured in media containing 4 and 10 mM of glucose. CSE (1 μg/mL) and CGA (10 μM) reinforced antioxidant defence and increased insulin secretion in response to glucose in beta cells stressed with streptozotocin (STZ). Since CGA concentration in CSE was of ≈ 0.1 nM it can be assumed that other antioxidants present in this particular extract may also contribute to the observed effect. In conclusion, here we provide evidence that CSE could be a new potential antidiabetic agent through its antioxidant actions and its ability to modulate insulin secretory function. Highlights: Coffee silverskin extract increases GPx activity in INS-1E cells. Coffee silverskin extract increases insulin secretion in response to glucose in INS-1E cells. Coffee silverskin extract protects against STZ induced oxidative stress in INS-1E cells. Coffee silverskin extract may reduce risk of diabetes through pancreas protection.