Determination of ractopamine in pork using a magnetic molecularly imprinted polymer as adsorbent followed by HPLC. (15th June 2016)

• Record Type: Journal Article
• Authors: Tang, Yiwei; Gao, Jingwen; Liu, Xiuying; Lan, Jianxing; Gao, Xue; Ma, Yong; Li, Min; Li, Jianrong
• Is Part Of: Food chemistry. Volume 201(2016)
• Page Start: 72
• Page End: 79
• Publication Date: 2016-06-15
• Subjects: Ractopamine -- Magnetic molecularly imprinted polymer -- Pork -- Extraction -- HPLC; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2016.01.070
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A magnetic functional monomer was prepared based on Fe3 O4 . A new magnetic imprinted polymer for RAC was prepared by surface imprinting method. A magnetic MIP-HPLC method for analysis of RAC in pork was developed. Abstract: A new magnetic molecularly imprinted polymers (MMIPs) for separation and concentration of ractopamine (RAC) were prepared using surface molecular imprinting technique with methacryloyl chloride as functional monomer and RAC as template. The MMIPs were characterized using transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating sample magnetometer. The results of re-binding experiments indicated that the MMIPs had fast adsorption kinetics and could reach binding equilibrium within 20 min, and the adsorption capacity of the MMIPs was 2.87-fold higher than that of the corresponding non-imprinted polymer. The selectivity of the MMIPs was evaluated according to its recognition to RAC and its analogues. The synthesized MMIPs were successfully applied to extraction, followed by high performance liquid chromatography to determine RAC in real food samples. Spiked recoveries ranged from 73.60% to 94.5%, with relative standard deviations of <11.17%.