Stability indicating simplified HPLC method for simultaneous analysis of resveratrol and quercetin in nanoparticles and human plasma. (15th April 2016)

• Record Type: Journal Article
• Authors: Kumar, Sandeep; Lather, Viney; Pandita, Deepti
• Is Part Of: Food chemistry. Volume 197:Part A(2016)
• Page Start: 959
• Page End: 964
• Publication Date: 2016-04-15
• Subjects: Resveratrol (PubChem CID: 445154) -- Quercetin (PubChem CID: 5280343) -- Poly(lactide-co-glycolide) (PubChem CID: 71391) -- Polyvinyl alcohol (PubChem CID: 11199); Forced degradation -- Isosbestic point -- PLGA nanoparticles -- Simultaneous quantification -- Polyphenols; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.11.078
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: An isosbestic point based HPLC method is developed and validated. The method is rapid, highly sensitive as well as economical. Stability studies of resveratrol and quercetin are established. The method is applicable on PLGA nanoparticles and human plasma. Abstract: Resveratrol and quercetin are well-known polyphenolic compounds present in common foods, which have demonstrated enormous potential in the treatment of a wide variety of diseases. Owing to their exciting synergistic potential and combination delivery applications, we developed a simple and rapid RP-HPLC method based on isosbestic point detection. The separation was carried out on phenomenex Synergi 4μ Hydro-RP 80A column using methanol: acetonitrile (ACN): 0.1% phosphoric acid (60:10:30) as mobile phase. The method was able to quantify nanograms of analytes simultaneously on a single wavelength (269 nm), making it highly sensitive, rapid as well as economical. Additionally, forced degradation studies of resveratrol and quercetin were established and the method's applicability was evaluated on PLGA nanoparticles and human plasma. The analytes peaks were found to be well resolved in the presence of degradation products and excipients. The simplicity of the developed method potentializes its suitability for routine in vitro and in vivo analysis of resveratrol and quercetin.