Genetic Diversity and Antibiotic Resistance Patterns of Staphylococcus Aureus Isolated from Leaf Vegetables in Korea. Issue 7 (27th May 2015)

• Record Type: Journal Article
• Authors: Hong, Jisoo; Kim, Yangkyun; Kim, Jonguk; Heu, Sunggi; Kim, Se‐ri; Kim, Kwang‐Pyo; Roh, Eunjung
• Is Part Of: Journal of food science. Volume 80:Issue 7(2015)
• Page Start: M1526
• Page End: M1531
• Publication Date: 2015-05-27
• Subjects: Food -- Periodicals; Food -- Research -- Periodicals; Food -- Periodicals; Research -- Periodicals; Levensmiddelen; Voeding; 664
• DOI: 10.1111/1750-3841.12909
• Languages: English
• ISSNs: 0022-1147

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p> <italic>Staphylococcus aureus</italic> is an important foodborne pathogen on global basis. The current study investigated the genetic patterns in <italic>S. aureus</italic> isolates from leaf vegetables (<italic>n</italic> = 53). Additional isolates from livestock (<italic>n</italic> = 31) and humans (<italic>n</italic> = 27) were compared with the leaf vegetable isolates. Genes associated with toxins, antibiotic resistance, and pulsed‐field gel electrophoresis (PFGE) patterns were analyzed. At least 1 enterotoxin‐encoding gene (<italic>sea</italic>, <italic>seb</italic>, <italic>sec</italic>, <italic>sed</italic>, and <italic>see</italic>) was detected in 11 of 53 (20.75%) leaf vegetable isolates. When the <italic>agr</italic> (accessory gene regulator) grouping was analyzed, <italic>agr</italic> II was the major group, whereas <italic>agr</italic> IV was not present in leaf vegetable isolates. All <italic>S. aureus</italic> isolates from leaf vegetables were resistant to more than one of the antibiotics tested. Nineteen of 53 (35.85%) isolates from leaf vegetables exhibited multidrug‐resistance, and 11 of these were MRSA (methicillin‐resistant <italic>S. aureus</italic>). A dendrogram displaying the composite types of <italic>S. aureus</italic> isolates from 3 origins was generated based on the combination of the toxin genes, <italic>agr</italic> genes, antibiotic resistance, and PFGE patterns. The isolates could be clustered into 8 major composite types. The genetic patterns of <italic>S. aureus</italic> isolates from leaf vegetables and humans were similar, whereas those from livestock had unique patterns. This suggests some <italic>S. aureus</italic> isolates from leaf vegetables to be of human origin.</p> </abstract>