Detection of Extended‐Spectrum β‐Lactamase and Plasmid‐Mediated Quinolone Resistance Determinants in Escherichia coli Isolates from Retail Meat in China. Issue 5 (11th April 2015)

• Record Type: Journal Article
• Authors: Yu, Tao; Jiang, Xiaobing; Fu, Kaifei; Liu, Biyun; Xu, Dong; Ji, Shengdong; Zhou, Lijun
• Is Part Of: Journal of food science. Volume 80:Issue 5(2015)
• Page Start: M1039
• Page End: M1043
• Publication Date: 2015-04-11
• Subjects: Food -- Periodicals; Food -- Research -- Periodicals; Food -- Periodicals; Research -- Periodicals; Levensmiddelen; Voeding; 664
• DOI: 10.1111/1750-3841.12870
• Languages: English
• ISSNs: 0022-1147

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The aim of this study was to investigate the presence of extended‐spectrum β‐lactamase (ESBL) and plasmid‐mediated quinolone resistance (PMQR) genes in <italic>Escherichia coli</italic> isolated from retail meat samples in Henan Province, China. <italic>E. coli</italic> isolates were detected in 179 of 645 (27.7%) retail meat samples. Resistance of these isolates to antimicrobials was commonly observed, with 78.2% of isolates resistant to streptomycin, 74.3% resistant to tetracycline and 54.2% resistant to trimethoprim/sulfamethoxazole. Of the 179 isolates, 30 (16.7%) expressed ESBL, with <italic>bla</italic><sub>TEM‐1</sub> (<italic>n</italic> = 17) and <italic>bla</italic><sub>CTX‐M‐14</sub> (<italic>n</italic> = 9) most commonly mediating the ESBL phenotype. PMQR genes were present in 14 isolates (7.8%), with <italic>qnr</italic> and <italic>aac(6′)‐Ib‐cr</italic> detected alone or in combination in nine (5.0%) and seven isolates (3.9%), respectively. The <italic>qnr</italic> genes detected included <italic>qnrS1</italic> (<italic>n</italic> = 5), <italic>qnrA1</italic> (<italic>n</italic> = 3), and <italic>qnrB4</italic> (<italic>n</italic> = 1). The <italic>qepA</italic> gene was absent among these isolates. CTX‐M‐14 was the most prevalent ESBL type among the PMQR‐positive isolates. The <italic>qnr</italic> and <italic>aac(6′)‐Ib‐cr</italic> genes were found to co‐reside and be co‐transferred with <italic>bla</italic><sub>CTX‐M‐14</sub> or <italic>bla</italic><sub>TEM‐1</sub> in five isolates. Our data suggest that retail meat may act as a reservoir for multi‐resistant <italic>E. coli</italic> and may facilitate the dissemination of resistance genes.</p> </abstract>