Inhibitory Activity of Paenibacillus polymyxa on the Biofilm Formation of Cronobacter spp. on Stainless Steel Surfaces. Issue 7 (14th June 2013)

• Record Type: Journal Article
• Authors: Yang, Soonwook; Kim, Seonhwa; Ryu, Jee‐Hoon; Kim, Hoikyung
• Is Part Of: Journal of food science. Volume 78:Issue 7(2013)
• Page Start: M1036
• Page End: M1040
• Publication Date: 2013-06-14
• Subjects: Food -- Periodicals; Food -- Research -- Periodicals; Food -- Periodicals; Research -- Periodicals; Levensmiddelen; Voeding; 664
• DOI: 10.1111/1750-3841.12168
• Languages: English
• ISSNs: 0022-1147

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The objective of this study was to control the survival or biofilm formation of <italic>Cronobacter</italic> spp. on stainless steel surfaces using <italic>Paenibacillus polymyxa</italic>. The antibacterial activity of a cell‐free culture supernatant (CFCS) of <italic>P. polymyxa</italic> against <italic>Cronobacter</italic> spp. was found to vary with <italic>P. polymyxa</italic> incubation time. Maximum activity occurred when <italic>P. polymyxa</italic> was incubated at 25 or 30 °C for 96 h. When the CFCS was introduced to <italic>Cronobacter</italic> spp. adhered to stainless steel strips at 25 °C for up to 72 h, the CFCS successfully inhibited <italic>Cronobacter</italic> biofilm formation. Additionally, stainless steel surfaces with a preformed <italic>P. polymyxa</italic> biofilm were exposed to <italic>Cronobacter</italic> spp. suspensions in PBS or 0.1% peptone water at 3, 5, or 7 log CFU/mL to facilitate its attachment. The <italic>Cronobacter</italic> population significantly decreased on this surface, regardless of inoculum level or carrier, when the <italic>P. polymyxa</italic> biofilm was present. However, the microbial population decreased within 6 h and remained unchanged thereafter when the surface was immersed in an inoculum suspended in 0.1% peptone water at 5 or 7 log CFU/mL. These results indicate that <italic>P. polymyxa</italic> is able to use a promising candidate competitive‐exclusion microorganism to control <italic>Cronobacter</italic> spp.</p> </abstract>