Development of Kaptive databases for Vibrio parahaemolyticus O- and K-antigen genotyping. Issue 5 (2nd May 2023)

• Record Type: Journal Article
• Authors: van der Graaf-van Bloois, Linda; Chen, Hongyou; Wagenaar, Jaap A.; Zomer, Aldert L.
• Is Part Of: Microbial genomics. Volume 9:Issue 5(2023)
• Publication Date: 2023-05-02
• Subjects: K-locus -- O-locus -- serotyping -- Vibrio parahaemolyticus -- whole-genome sequencing -- genotyping; Microbial genomics -- Periodicals; 572.8629
• DOI: 10.1099/mgen.0.001007
• Languages: English
• ISSNs: 2057-5858

Abstract

Abstract : Vibrio parahaemolyticus is an important food-borne human pathogen and presents immunogenic surface polysaccharides, which can be used to distinguish problematic and disease-causing lineages. V. parahaemolyticus is divided in 16 O-serotypes (O-antigen) and 71 K-serotypes (K-antigen). Agglutination tests are still the gold standard for serotyping, but many V. parahaemolyticus isolates are not typable by agglutination. An alternative for agglutination tests is genotyping using whole-genome sequencing data, by which K- and O- genotypes have been curated and identified previously for other clinically relevant organisms with the software tool Kaptive . In this study, V. parahaemolyticus isolates were serotyped and sequenced, and all known and several novel O- and K-loci were identified. We developed Kaptive databases for all O- and K-loci after manual curation of the loci. In our study, we could genotype the O- and K-loci of 98 and 93 % of the genomes, respectively, with a Kaptive confidence score higher than 'none'. The newly developed Kaptive databases with the identified V. parahaemolyticus O- and K-loci can be used to identify the O- and K-genotypes of V. parahaemolyticus isolates from genome sequences.