Metabolites profiling of protein enriched oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm.) grown on oil palm empty fruit bunch substrate. (1st May 2023)

• Record Type: Journal Article
• Authors: Lee, Aubrey Mei Li; Chin, Clament Fui Seung; Seelan, Jaya Seelan Sathiya; Chye, Fook Yee; Lee, Huei Hong; Rakib, Mohd. Rashid Mohd.
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 181(2023)
• Publication Date: 2023-05-01
• Subjects: Agro wastes -- Empty fruit bunches -- Mycoprotein -- Free amino acids -- UPLC-QToF MS; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2023.114731
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: This study aimed to compare the nutritional composition and metabolites profiles of the Pleurotus ostreatus oyster mushroom grown on oil palm empty fruit bunch (EFB) and rubber wood sawdust (RWS) substrates. The mushrooms were prepared for proximate analysis, beta-glucan content, and metabolites profiling using ultra high performance liquid chromatography-quadrupole time of flights mass spectrometer (UHPLC-QToF). Proximate analysis showed significantly higher crude protein, and beta-glucan contents ( p < 0.05) were recovered from the mushrooms grown on EFB substrate as compared to those grown on RWS. Metabolite profiling of hot water extracts showed that three lipids (PE (17:1/0:0), PE (16:1/0:0), and hydroxyoctadecadienoic), two amino acids (acetyl-leucine, and threonic acid), and one polysaccharide (4-O-beta-d -galactopyranosyl-beta-d -xylopyranose) were present at a relatively higher level (maximum fold change >10) in mushrooms grown on EFB. Our results suggest that the metabolites of the mushroom cultivated on EFB substrate were altered. The innovative use of palm oil waste as mushroom cultivation substrate exerts the concept of waste-to-wealth, promoting Sustainable Palm Oil Initiative (SPOI), and enhancing food security through the production of high-protein mushrooms. Highlights: Mushrooms grown on EFB substrate has higher crude protein, and beta glucan. Three lipids, two amino acids, and one polysaccharide were relatively higher in EFB-grown mushroom. The valorisation of EFB as mushroom substrate helps to improve food security and build food system resilience.