Characterization, chemical modification and bioactivities of a polysaccharide from Stropharia rugosoannulata. (May 2023)

• Record Type: Journal Article
• Authors: Jin, Mingzhi; Zhang, Wenqing; Zhang, Xinmiao; Huang, Qianli; Chen, Hui; Ye, Ming
• Is Part Of: Process biochemistry. Volume 128(2023)
• Page Start: 30
• Page End: 39
• Publication Date: 2023-05
• Subjects: Stropharia rugosoannulata -- Polysaccharide -- Chemical characterization -- Chemical modification -- Antioxidant activity -- Enzyme inhibitory activity; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2023.02.008
• Languages: English
• ISSNs: 1359-5113

Abstract

Abstract: This study evaluates the chemical structure of a heteropolysaccharide (SP-1a) from the fruiting bodies of Stropharia rugosoannulata and the antioxidant activity and enzyme inhibitory activity of its derivatives. The results showed that SP-1a (2.29 ×10 4 Da) contained fucose, glucose and galactose, and possessed the backbone of [→3)-α-D -Glc p -(1→] and [→3, 6)-α-D -Glc p -(1→] with side chains [→6)-α-D -Gal p -(1→] and T-α-D -Gal p . Furthermore, carboxymethylated and phosphorylated modification of SP-1a (named as CSP-1a and PSP-1a) had good thermal stability and strong rehydration. Moreover, CSP-1a displayed stable DPPH scavenging ability (46.77%) and reducing power ability (0.434), as well as α-glucosidase inhibitory activity (60.98%) and α-amylase inhibitory activity (67.20%). Besides, PSP-1a presented significant hydroxyl radical scavenging ability (94.18%). The findings suggested that appropriate chemical modifications can improve the physicochemical properties and biological activities of polysaccharides from Stropharia rugosoannulata, providing hints and theoretical supports for further development of corresponding drug or food ingredients. Graphical Abstract: ga1 Highlights: The structure of polysaccharide (SP-1a) from S. rugosoannulata was established. SP-1a was carboxymethylated and phosphorylated successfully. Carboxymethylated SP-1a showed stronger antioxidant and enzyme inhibitory activities. Phosphorylated SP-1a presented significant hydroxyl radical scavenging ability.