Potency testing of up to sixteen cannabinoids in hemp-infused edibles using liquid chromatography diode array detector with optional confirmation of identity by electrospray ionization time-of-flight mass spectrometry. (15th August 2023)

• Record Type: Journal Article
• Authors: Song, Liguo; Meyer, Grant; Adejumo, Emmanuel; Jovanovich, Emily; LeBlanc, Lindsey; Provis, Jake
• Is Part Of: Food chemistry. Volume 417(2023)
• Publication Date: 2023-08-15
• Subjects: Cannabinoid -- Hemp -- Edible -- Liquid chromatography -- Diode array detector -- Electrospray ionization time-of-flight mass spectrometry; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2023.135819
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: 16 cannabinoids analyzed in 18 min by liquid chromatography diode-array detector. Broad application to matrices such as drink, gummy, coffee, snack and pet treat. Samples or their uniform water-suspensions extracted by MeOH under homogenization. Cannabinoid recovery from each edible tracked in real time by spiking abnormal CBD. Good method specificity confirmed by time-of-flight mass spectrometry. Abstract: A LC-DAD method for potency testing of up to sixteen cannabinoids has been developed, validated, and applied for analysis of twenty hemp-infused edibles encompassing a broad range of complex matrices. The method was validated according to ISO 17025 guidelines and met requirements. Samples or their uniform water-dispersions were extracted by methanol under homogenization through pulverization and/or ultrasonication. By spiking abnormal cannabidiol, a cannabinoid not naturally present in hemp, into each sample, extraction recovery was tracked in real time, obtaining 90 to 108% in triplicates with relative standard deviations of 0.5 to 6.5%. The linear calibration range was between 0.008 and 10% (w/w) for each cannabinoid using a 250 µg/mL solution of hemp-infused edibles, except for drinks (sparkling water and tea), where it was between 0.0008 and 1% (w/w) using a 2.5 mg/mL solution. ESI/TOFMS confirmed a good method specificity, i.e., without any false positive identification of individual cannabinoid.