Effects of high dairy protein intake and vitamin D supplementation on body composition and cardiometabolic markers in 6–8-y-old children—the D-pro trial. Issue 4 (7th January 2022)

• Record Type: Journal Article
• Authors: Thams, Line; Stounbjerg, Nanna G; Hvid, Lars G; Mølgaard, Christian; Hansen, Mette; Damsgaard, Camilla T
• Is Part Of: American journal of clinical nutrition. Volume 115:Issue 4(2022)
• Page Start: 1080
• Page End: 1091
• Publication Date: 2022-01-07
• Subjects: pediatric -- cholecalciferol -- DXA -- BMI -- FMI -- FFMI -- cholesterol -- blood lipids -- cardiovascular -- milk protein; Diet therapy -- Periodicals; Nutrition -- Periodicals; Dietetics -- Periodicals; 613.205
• DOI: 10.1093/ajcn/nqab424
• Languages: English
• ISSNs: 0002-9165

Abstract

ABSTRACT: Background: Increasing evidence suggests that prevention of lifestyle diseases should begin early. Dairy protein and vitamin D can affect body composition and cardiometabolic markers, yet evidence among well-nourished children is sparse. Objectives: We investigated combined and separate effects of high dairy protein intake and vitamin D on body composition and cardiometabolic markers in children. Methods: In a 2 × 2–factorial, randomized trial, 200 white, Danish, 6–8-y-old children substituted 260 g/d dairy in their diet with high-protein (HP; 10 g protein/100 g) or normal-protein (NP; 3.5 g protein/100 g) yogurt and received blinded tablets with 20 µg/d vitamin D3 or placebo for 24 wk during winter. We measured body composition (by DXA), blood pressure, and fasting blood glucose, insulin, C-peptide, and lipids. Results: In total, 184 children (92%) completed the study. Baseline median (25th–75th percentile) dairy protein intake was median: 3.7 (25th–75th percentile: 2.5–5.1) energy percentage (E%) and increased to median: 7.2 (25th–75th percentile: 4.7–8.8) E% and median: 4.2 (25th–75th percentile: 3.1–5.3) E% with HP and NP. Mean ± SD serum 25-hydroxyvitamin D concentration changed from 81 ± 17 to 89 ± 18 nmol/L and 48 ± 13 nmol/L with vitamin D and placebo, respectively. There were no combined effects of dairy protein and vitamin D, except for plasma glucose, with the largest increase in the NP–vitamin D group ( P interaction = 0.005). There were smaller increases in fat mass index ( P = 0.04) with HP than with NP, and the same pattern was seen for insulin, HOMA-IR, and C-peptide (all P = 0.06). LDL cholesterol was reduced with vitamin D compared with placebo ( P < 0.05). Fat-free mass and blood pressure were unaffected. Conclusions: High compared with normal dairy protein intake hampered an increase in fat mass index. Vitamin D supplementation counteracted the winter decline in 25-hydroxyvitamin D and the increase in LDL cholesterol observed with placebo. This study adds to the sparse evidence on dairy protein in well-nourished children and supports a vitamin D intake of ∼20 µg/d during winter. This trial was registered at clinicaltrials.gov as NCT03956732.