Identification and quantification of phytoprostanes and phytofurans in Acacia cyanophylla Lindl. seeds. (October 2022)

• Record Type: Journal Article
• Authors: Mersni, Marwa; Zhou, Bingqing; Reversat, Guillaume; Yangui, Islem; Khouja, Mohamed Larbi; Guy, Alexandre; Oger, Camille; Galano, Jean-Marie; Durand, Thierry; Messaoud, Chokri; Vigor, Claire
• Is Part Of: Food Chemistry Advances. Volume 1(2022)
• Publication Date: 2022-10
• Subjects: Acacia cyanophylla Lindl. seeds -- Phytoprostanes -- Phytofurans -- Micro-LC-MS/MS -- Climatic conditions; PhytoPs Phytoprostanes -- PhytoFs Phytofurans -- ALA α-linolenic acid -- PUFA Polyunsaturated fatty acid -- ROS reactive oxygen species -- LC-MS/MS liquid chromatography−mass spectrometry -- HPLC high-pressure liquid chromatography -- SPE solid phase extraction -- IS Internal Standard -- MRM monitoring reaction mode -- QTRAP triple-quadrupole combined to ion trap mass spectrometer -- LOD the limit of detection -- LOQ the limit of quantification -- EY extraction yield -- ME matrix effect -- SD standard deviation -- PCA principal component analysis; 664
• DOI: 10.1016/j.focha.2022.100077
• Languages: English
• ISSNs: 2772-753X

Abstract

Highlights: A new liquid mass chromatography tandem mass method for identification of oxylipins. Acacia cyanophylla seeds is a source of bioactive metabolites with medicinal value. Influence of ecological factors in phytoprostanes and phytofurans profiles. Abstract: Phytoprostanes and phytofurans are derived from peroxidation of α-linolenic acid (ALA) the main polyunsaturated fatty acid (PUFA) found in higher plants. These compounds are considered as excellent biomarkers of oxidative degradation and have demonstrated a wide range of biological activities (e.g. protection of neuronal cells against stress, anti-inflammatory). In the present work, these new active oxylipins, were detected for the first time in Acacia cyanophylla seeds collected in Tunisia from 7 ecotypes, using LC-MS/MS technology. The main PhytoP found was ent -9-L1 -PhytoP, ranged from 3.30 ng/g (N-OB ecotype) to 19.14 ng/g (B-ZO ecotype). Three PhytoFs were detected with significantly higher values than PhytoPs. Multivariate analyses indicated a significant differentiation between compounds attributed to variation in climatic factors. This first complete PhytoP and PhytoF profile shows that Acacia cyanophylla Lindl. seeds could be as a new source of bioactive compounds for therapeutic and pharmaceutical applications. Graphical abstract: Image, graphical abstract