Competitive fluorescent immunosensor based on catalytic hairpin self-assembly for multiresidue detection of organophosphate pesticides in agricultural products. (1st July 2023)

• Record Type: Journal Article
• Authors: Wang, Yuanshang; Abd El-Aty, A.M.; Wang, Shanshan; Cui, Xueyan; Zhao, Jing; Lei, Xingmei; Xu, Lingyuan; She, Yongxin; Jin, Fen; Eun, Jong-Bang; Shim, Jae-Han; Wang, Jing; Jin, Maojun; Hammock, Bruce D.
• Is Part Of: Food chemistry. Volume 413(2023)
• Publication Date: 2023-07-01
• Subjects: Catalyzed hairpin self-assembly -- Fluorescent immunosensors -- Multiresidue detection -- Organophosphate pesticides -- Gold nanoparticles; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2023.135607
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Dual signal amplification was achieved by combining immunoassay and nucleic acid amplification. The method could occur at a constant temperature without enzyme catalysis. The detection limit was as low as 0.012, 0.0057, and 0.0074 ng/mL. Signals were detected through different fluorescent groups modified on DNA strands. The designed sensor leads to a new direction for trace analysis of multiple pesticide residues. Abstract: Simple and rapid multiresidue trace detection of organophosphate pesticides (OPs) is extremely important for various reasons, including food safety, environmental monitoring, and national health. Here, a catalytic hairpin self-assembly (CHA)-based competitive fluorescent immunosensor was developed to detect OPs in agricultural products, involving enabled dual signal amplification followed by a CHA reaction. The developed method could detect 0.01–50 ng/mL triazophos, parathion, and chlorpyrifos, with limits of detection (LODs) of 0.012, 0.0057, and 0.0074 ng/mL, respectively. The spiked recoveries of samples measured using this assay ranged from 82.8 % to 110.6 %, with CV values ranging between 5.5 % and 18.5 %. This finding suggests that the CHA-based competitive fluorescent immunosensor is a reliable and accurate method for detecting OPs in agricultural products. The results correlated well with those obtained from the liquid chromatography-tandem mass spectrometry (LC–MS/MS) method, indicating that the CHA-based biosensor is able to accurately detect OPs and can be used as a reliable alternative to the LC–MS/MS method. Additionally, the CHA-based biosensor is simpler and faster than LC–MS/MS, which makes it a more practical and cost-effective option for the detection of OPs. In summary, the CHA-based competitive fluorescent immunosensor can be considered a promising approach for trace analysis and multiresidue determination of pesticides, which can open up new horizons in the fields of food safety, environmental monitoring, and national health.