Functional, structural properties and interaction mechanism of soy protein isolate nanoparticles modified by high-performance protein-glutaminase. (May 2023)

• Record Type: Journal Article
• Authors: Zheng, Nan; Long, Mengfei; Zhang, Zehua; Osire, Tolbert; Zan, Qijia; Zhou, Huimin; Du, Shuang; Xia, Xiaole
• Is Part Of: Food hydrocolloids. Volume 139(2023)
• Publication Date: 2023-05
• Subjects: Soy protein isolate nanoparticles -- Protein-glutaminase -- Protein-protein interaction -- Molecular dynamics simulation -- Functional properties -- Structural characteristics; Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2023.108594
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: Investigating the changes in the structural and functional properties of soy protein isolate (SPI) modified by high-performance protein-glutaminase (PG) and understanding their interactions are crucial for the application of SPI nanoparticles. Herein, the thermal stability and activity of PG were evolved by computer-assisted rational design strategy, generating the best variant Z15 (N16M/N20L/Q21H/K48R/S81E/T113E) with a 4.65-fold improved activity (75.57 U/mg) and increased thermal stability (Δ T m = 4.6 °C), one of the best performing PG. SPI treated with Z15 showed better solubility, foaming, and emulsifying properties than the wild type PG-deamidation, which were increased by 1.25-fold, 45.45%, and 38.95%, respectively. Furthermore, SPI treated with Z15 exhibited lower hardness and higher cohesiveness. By systematically analyzing the structural characteristics, SPI nanoparticles possessed a coarse, loose, and porous microstructure. Molecular dynamics simulations suggested that the Glu81 and Glu113 residues of Z15 contributed the most to the stabilization interaction in β-conglycinin-Z15 complexes. These results could provide theoretical guidance for the potential application of SPI nanoparticles in sustainable green plant-based foods industries. Graphical abstract: Image 1 Highlights: Z15 exhibited great specific activity (75.57 U/mg) and thermostability ( T m 72.4 °C). SPI nanoparticles treated with Z15 showed dramatic increase in functional properties. SPI nanoparticles treated with Z15 presented excellent homogeneity and stability. Glu81 and Glu113 were key residues with big energy contribution in 7S-Z15 complexes.