Structural characterization of a heteropolysaccharide from the fruit of Crataegus pinnatifida and its bioactivity on the gut microbiota of immunocompromised mice. (1st July 2023)

• Record Type: Journal Article
• Authors: Jing, Yongshuai; Zhang, Yameng; Yan, Meng; Zhang, Ruijuan; Hu, Beibei; Sun, Shiguo; Zhang, Danshen; Zheng, Yuguang; Wu, Lanfang
• Is Part Of: Food chemistry. Volume 413(2023)
• Publication Date: 2023-07-01
• Subjects: Crataegus pinnatifida -- Polysaccharides -- Structural characterization -- Immunoregulatory activity -- Gut microbiota; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2023.135658
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A novel polysaccharide (CPP) was isolated from the fruit of Crataegus pinnatifida. The preliminary structural characterization of CPP was conducted for the first time. CPP could improve the diversity of gut microbiota and the release of immune factors. CPP had the potential as an immune and a gut microbiota community regulator. Abstract: Crataegus pinnatifida is a common food in China, Europe and North America. In order to confirm polysaccharide was the material basis for C. pinnatifida to exert immune regulation. A polysaccharide (CPP) with a molecular weight of 13.58 kDa was isolated from C. pinnatifida . The structure of CPP was determined to be a backbone composed of → 3, 5)-α- l -Ara f -(1→, with two branches consisting of → 4)-α- d -Gal p -(1 → and → 5)-α- l -Ara f -(1→, with α- l -Ara f and α- d -Man p as the terminal unit. CPP (10 ∼ 500 μg/mL) could promote the secretion of nitric oxide, interleukin-2, interleukin-6 and tumor necrosis factor-α in vitro . CPP could significantly restore the body weight of immunosuppressive mice and improve the immune organ index and interleukin-2, interleukin-6, and tumor necrosis factor-α secretion. In addition, CPP increased the abundance of Bacteroidetes and Verrucomicrobia and decreased the abundance of Proteobacteria at the phylum level. So CPP can regulate the gut microbiota and play an important role in immune regulation.