Detection of antimicrobial residues in animal manure by a microbiological screening methodology: A non-invasive tool in animal production. (June 2023)

• Record Type: Journal Article
• Authors: Astudillo, Diego; Pokrant, Ekaterina; Bravo, Camila; Ríos, Alejandra; Navarrete, María José; Maddaleno, Aldo; Maturana, Matías; Flores, Andrés; Guzmán, Miguel; Hidalgo, Héctor; Zayas, Caridad; Lapierre, Lisette; Cornejo, Javiera
• Is Part Of: Food control. Volume 148(2023)
• Publication Date: 2023-06
• Subjects: Antimicrobials -- Residues -- Screening method -- Animal manure -- Non-invasive; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2023.109649
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Antimicrobial residues are of great importance in food production because of the potential for negative consequences on human health, such as antimicrobial resistance. A large percentage of these drugs are excreted and persist in animal waste. Therefore, animal waste could be used as a non-invasive matrix for antimicrobial analysis, and a tool to control the use of antimicrobials in animal production. A new microbiological screening method for the detection of antimicrobials for six classes in manure was developed, implemented, validated, and verified in vivo . Validation was performed by assessing the specificity, CCβ, stability and ruggedness according to (EU) 2021/808. The in vivo verification was performed in broiler chickens. Six birds were treated per group, one for each antimicrobial class. Manure samples were analysed by the screening methodology and HPLC-MS/MS, as confirmation. The implemented six plate screening was specific to detect all antimicrobials tested. A CCβ of 200, μg kg −1 was determined for macrolides, of 300 μg kg −1 for β-lactams and sulphonamides, 400 μg kg −1 for tetracyclines and lincosamides, and 500 μg kg −1 for, quinolones. positive samples were confirmed by HPLC-MS/MS. The applicability of the method was tested on pig manure, in these samples a higher number of false positives were obtained with respect to chicken droppings. In conclusion, this new screening method presented performance characteristics that demonstrate utility for the intended analytical applications, ensuring traceability of the results. Highlights: New microbiological screening method for antimicrobial detection in animal manure. Validation of the screening method for detecting six different antimicrobial classes. In vivo verification of the methodology was performed in broiler manure. The applicability of the method on pig manure field samples was determined.