Tannic acid-derived selective capture of bacteria from apple juice. (30th June 2023)

• Record Type: Journal Article
• Authors: Kang, Yi; Li, Min; Han, Yaru; Sun, Hao; Dan, Jie; Liang, Yanmin; Zhang, Qiuping; Su, Zehui; Yue, Tianli; Wang, Jianlong; Zhang, Wentao
• Is Part Of: Food chemistry. Volume 412(2023)
• Publication Date: 2023-06-30
• Subjects: Adsorption -- Gram-positive bacteria -- Independent gradient model -- Magnetic separation -- Tannic acid; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2023.135539
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Tannic acid functionalized virus-like Fe3 O4 captures Gram-positive bacteria quickly. Peptidoglycan fragments interact with tannic acid. Viral-like structure enhances bacterial attachment and adhesion. Magnetic attraction separates bacteria from apple juice, preventing bacterial residue. vFe3 O4 -TA didn't affect juice's SSC, color, titratable acidity and transmittance. Abstract: Given the enormous burden pathogens pose on human health, rapid capture and removal of bacteria for sterilization or further bacterial detection is essential. Herein, tannic acid-functionalized virus-like Fe3 O4 (vFe3 O4 -TA) was established for bacterial enrichment. We investigated the ability of vFe3 O4 -TA to capture Gram-negative bacteria ( E. coli, S. flex and S. typhi ) and Gram-positive bacteria ( S. aureus, MRSA and LM), respectively. Compared to the capture efficiency of <15 % for Gram-negative bacteria, vFe3 O4 -TA showed excellent selectivity and efficiency in isolating Gram-positive bacteria with >87 % removal efficiency. GFN-xTB semiempirical quantum chemical calculations revealed that the selective recognition originates from the high affinity between TA and peptidoglycan. Without impacting ingredients, the TA-mediated trapper also shows excellent ability to distinguish Gram-positive bacteria in juice samples. These results are expected to reveal the interaction of TA with bacteria, and inaugurate a potential natural safe tool for food safety control, medical treatment and environmental remediation.