In vitro antioxidant and anti-denaturation effects of Buglossoides purpurocaerulea (L.) I. M. Johnst. fruit extract. Issue 6 (19th March 2023)

• Record Type: Journal Article
• Authors: Marrelli, Mariangela; Amodeo, Valentina; Puntillo, Domenico; Statti, Giancarlo; Conforti, Filomena
• Is Part Of: Natural product research. Volume 37:Issue 6(2023)
• Page Start: 1012
• Page End: 1015
• Publication Date: 2023-03-19
• Subjects: Boraginaceae -- bovine serum albumin -- Lithospermum; rosmarinic acid -- phenols; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2022.2096607
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Fruits from Buglossoides purpurocaerulea (L.) I. M. Johnst. were extracted with methanol through maceration. The phytochemical composition of obtained raw extract was investigated with gas chromatography-mass spectrometry (GC-MS) and high-performance thin layer chromatography (HPTLC) analyses. The total phenolic and flavonoid contents were also quantified and in vitro antioxidant activity was assessed using the DPPH assay and the β-carotene bleaching test. In order to verify the anti-arthritic potential, the raw extract was evaluated for its anti-denaturation effect on heat-treated bovine serum albumin (BSA), chosen as a protein model. Linoleic and α-linolenic acids were the most abundant identified apolar constituents. HPTLC analyses allowed the identification of rosmarinic acid. The sample showed a concentration-dependent antioxidant activity and it was also proven to be effective in preventing the heat-induced denaturation of BSA, with an IC50 value equal to 15.7 ± 0.5 µg/mL. Graphical Abstract: UF0001