Comparison between cage and free-range egg production on microbial composition, diversity and the presence of Salmonella enterica. (August 2021)

• Record Type: Journal Article
• Authors: Wilson, Annaleise; Chandry, P. Scott; Turner, Mark S.; Courtice, Jodi M.; Fegan, Narelle
• Is Part Of: Food microbiology. Volume 97(2021)
• Publication Date: 2021-08
• Subjects: Culture-independent -- Bacterial microbiota -- Salmonella enterica -- Eggs -- Food safety -- Farm environmental bacteria; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2021.103754
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: The microbial composition of the food production environment plays an important role in food safety and quality. This study employed both 16 S rRNA gene sequencing technology and culture-based techniques to investigate the bacterial microbiota of an egg production facility comprising of both free-range and conventional cage housing systems. The study also aimed to detect the presence of Salmonella enterica and determine whether its presence was positively or negatively associated with other taxa. Our findings revealed that microbiota profiles of free-range and cage houses differ considerably in relation to the relative abundance and diversity with a number of taxa unique to each system and to individual sampling sites within sheds. Core to each housing system were known inhabitants of the poultry gastrointestinal tracts, Romboutsia and Turicibacter, as well as common spoilage bacteria. Generally, free-range samples contained fewer taxa and were dominated by Staphylococcus equorum, differentiating them from the cage samples. Salmonella enterica was significantly associated with the presence of a taxa belonging to the Carnobacteriaceae family. The results of this study demonstrate that the diversity and composition of the microbiota is highly variable across egg layer housing systems, which could have implications for productivity, food safety and spoilage. Highlights: The microbiota of two egg farm housing systems was characterised using 16S amplicon sequencing. Cage housing samples had significantly higher alpha diversity scores compared to free range housing samples. Staphylococcus e quorum was the dominant bacterium found in free range housing samples. Spoilage bacteria and gastrointestinal bacteria were the dominant bacteria found cage housing samples. Salmonella enterica was not identified in Salmonella -positive samples using 16 S amplicon metagenomics.