Isolation, host specificity and genetic characterization of Campylobacter specific bacteriophages from poultry and swine sources. (August 2021)

• Record Type: Journal Article
• Authors: Nafarrate, Ibai; Mateo, Estibaliz; Miranda-Cadena, Katherine; Lasagabaster, Amaia
• Is Part Of: Food microbiology. Volume 97(2021)
• Publication Date: 2021-08
• Subjects: Campylophage -- Biocontrol -- PFGE -- RFLP -- RAPD-PCR -- Lytic spectrum; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2021.103742
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: The isolation and characterization of 304 Campylobacter specific bacteriophage isolates from broiler and swine sources is reported in this study. Genome size characterization determined by PFGE classified these isolates, called CAM1-CAM304, within the campylophages group II (n = 18) and group III (n = 286). Host range analyses showed a high host specificity and similar lytic spectrum among isolates of the same group. Campylophages of group II infected C. jejuni, C. coli and even a C. fetus strain whereas those of group III only infected C. jejuni strains. The most promising 59 campylophage candidates were selected according to their lytic activity and their genetic diversity was analyzed by RFLP using Smi I and Hha I endonucleases for group II and III campylophages, respectively. Moreover, RAPD-PCR technique was for the first time assessed in the genetic characterization of campylophages and it was shown to be effective only for those of group II. Bacteriophage isolates grouped in a same genotype displayed different host ranges, therefore, 13 campylophages of group II and eight of group III were differentiated considering all the approaches assayed. An in-depth analysis of these bacteriophages will be performed to confirm their promising potential for the biocontrol of Campylobacter within the farm to fork process. Highlights: RAPD-PCR technique is effective for campylophage genetic diversity characterization. Campylophage isolates grouped into the same genotype display different host ranges. Campylophages of group II showed a broader lytic spectrum than those of group III. Campylophages of group II are able to infect C. coli, C. jejuni and C. fetus .