Isolation and characterization of a novel Salmonella bacteriophage JNwz02 capable of lysing Escherichia coli O157:H7 and its antibacterial application in foods. (1st January 2023)

• Record Type: Journal Article
• Authors: Zhang, Hui-Zhen; Shu, Mei; Yang, Wen-Yu; Pan, Hong; Tang, Men-Xuan; Zhao, Yuan-Yang; Zhong, Chan; Wu, Guo-Ping
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 173(2023)
• Publication Date: 2023-01-01
• Subjects: T5-like bacteriophage -- Cross-infecting E. coli O157:H7 -- Genome sequence -- Characteristics -- Biocontrol agent; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2022.114251
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: The topic of bacteriophage as an alternative to antibiotics to control pathogenic bacteria has attracted attention. Here, a novel virulent Salmonella bacteriophage JNwz02 capable of cross-infecting E. coli O157:H7 was isolated and characterized. The complete genome of JNwz02 was composed of 114390 bp double-stranded DNA molecules encoding 164 open reading frames (ORFs) with a G + C content of 40.22%. JNwz02 belongs to the Siphoviridae family and has high homology with T5-type phages. The eclipse period and latency of JNwz02 infection with Salmonella were 10 min and 10–15 min, respectively. The burst size and adsorption rate constant K were 200 plaque-forming unit (PFU)/colony-forming unit (CFU) and 6.71 × 10 −9 ml/min, respectively. JNwz02 was relatively stable in the range of temperature (30°C-50 °C) and pH (2–11). The viable Salmonella and E. coli O157:H7 were significantly reduced in ready-to-eat duck neck meat and milk after treatment with JNwz02 at 4 °C for 2 d. The results suggested that JNwz02 has potential for application against Salmonella and E. coli in food. Graphical abstract: Image 1 Highlights: A novel Salmonella phage JNwz02 capable of cross-infecting E. coli O157:H7 was isolated and identified. JNwz02 contains 114390-bp double-stranded DNA encoding 164 ORFs. JNwz02 belongs to the Siphoviridae family and has high homology with T5-type phages. JNwz02 could significantly reduce Salmonella and E. coli O157: H7 in foods.