Lactobacillus plantarum CY 1-1: A novel quorum quenching bacteria and anti-biofilm agent against Aeromonas sobria. (February 2021)

• Record Type: Journal Article
• Authors: Lv, Xinran; Cui, Tianqi; Du, Hong; Sun, Mengtong; Bai, Fengling; Li, Jianrong; Zhang, Defu
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 137(2021)
• Publication Date: 2021-02
• Subjects: Lactobacillus plantarum strain CY 1-1 -- Quorum quenching -- Degradation -- Anti-Biofilm -- Aeromonas sobria; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2020.110439
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Aeromonas sobria is a Gram-negative pathogen with numerous virulence factors that lead to diseases in aquacultures. The production of virulence factors and biofilm formation in A. sobria are regulated by a quorum sensing (QS) system. In this study, we isolated bacterial strain CY 1-1 from pickles with strong quorum quenching activity by screening 154 strains of lactic acid bacteria. Subsequently, we identified it as Lactobacillus plantarum by 16s rRNA sequencing analysis and observed a close to 100% degradation rate for A. sobria -derived N -acyl homoserine lactone. The minimum inhibitory concentration (MIC) of L. plantarum CY 1-1 crude extract (CE) was 4.0 mg/mL. Sub-MICs of 1.0, 2.0, and 3.0 mg/mL L . plantarum CY 1-1 CE showed increased degradation rates for QS-mediated violacein production by Chromobacterium violaceum CV026 and reduced biofilm formation (33.03–81.93%), preformed biofilm (22.01–66.23%), extracellular proteases (17.48–50.56%), haemolytic activity (18.84–53.77%), siderophore (27.49–67.86%), and swarming and swimming (52.49–100%) in a dose-dependent manner. Scanning electron microscopy images indicated that L. plantarum CY 1-1 CE destroyed biofilm structure and reduced the thickness of preformed biofilm. Thus, L. plantarum CY 1-1 CE is a promising QS inhibitor and anti -biofilm agent for improving food safety. Highlights: L. plantarum CY 1-1 blocked the QS systems of A. sobria . CY 1-1 inhibited QS-regulated phenotypes in A. sobria . CY 1-1 reduced biofilm and virulence factor formation and removed preformed biofilm. CY 1–1 may act as a novel QS-based agent for improving food safety.