Assembling cyanidin-3-O-glucoside by using low-viscosity alginate to improve its in vitro bioaccessibility and in vivo bioavailability. (1st September 2021)

• Record Type: Journal Article
• Authors: Zou, Chao; Huang, Ling; Li, Donghui; Ma, Yu; Liu, Yixiang; Wang, Yanbo; Cao, Min-Jie; Liu, Guang-Ming; Sun, Lechang
• Is Part Of: Food chemistry. Volume 355(2021)
• Publication Date: 2021-09-01
• Subjects: Cyanidin-3-O-glucoside -- Alginate -- Molecular self-assembly -- Gastrointestinal digestion -- Molecular docking -- Bioaccessibility; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.129681
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: C3G and LVA can be self-assembled into a complex through hydrogen bonding at pH 3.0. The optimized binding mass ratio of C3G-LVA was 3:1. The binding efficiency of C3G was 84.2% under the optimized conditions. The LVA assembly improved the bioaccessibility of C3G during the simulated digestive process. The LVA assembly increased the plasma concentration of C3G by 27.4%. Abstract: In this work, an enteric soluble alginate was proposed to improve the absorption efficiency of cyanidin-3-O-glucoside (C3G) through molecular self-assembly. Under the optimized conditions, the obtained low-viscosity alginate (LVA) was released completely during the simulated gastrointestinal digestion and an LVA–C3G complex with 84.2% binding efficiency was acquired. Fourier transform infrared spectroscopy displayed that the characteristic spectrum of C3G had disappeared after the LVA conjugation. Furthermore, based on the analysis of scanning electron microscopy and differential scanning calorimetry, a porous network structure and the shifted endothermic peak in the thermograms were observed, further confirming the formation of a complex between LVA and C3G. The results of simulated gastrointestinal digestion reveal that the LVA assembly significantly ( p < 0.05) improved the bioaccessibility of C3G. Correspondingly, the C3G level in mouse plasma was increased by 27.4% in the C3G-LVA group. This suggests the suitability of LVA as an oral delivery vehicle for dietary anthocyanins.