Enhanced production of γ-aminobutyric acid (GABA) using Lactobacillus plantarum EJ2014 with simple medium composition. (February 2021)

• Record Type: Journal Article
• Authors: Park, Su Jeong; Kim, Dong Hyun; Kang, Hye Jee; Shin, Minhye; Yang, Soo-Yeon; Yang, Jungwoo; Jung, Young Hoon
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 137(2021)
• Publication Date: 2021-02
• Subjects: γ-Aminobutyric acid -- Lactobacillus plantarum -- Glutamate decarboxylase -- Monosodium l-glutamate -- Yeast extract -- Media optimization; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2020.110443
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: γ-Aminobutyric acid (GABA) is an amino acid not present in proteins. GABA participates in a variety of physiological functions and has received significant attention in the medical and food industries. In this study, GABA production was investigated using Lactobacillus plantarum . We designed a synthetic medium consisting of the simplest medium components such as yeast extract (nitrogen source), glucose (carbon source), and monosodium l -glutamate (MSG; substrate for GABA) and optimized their proportions to efficiently produce GABA. As a result, 19.8 g/L GABA was obtained under an optimal synthetic medium composition of 100 g/L yeast extract, 10 g/L glucose, and 2.25% MSG, which is the highest among previous studies using L. plantarum . Interestingly, 159.7% of theoretical maximum yield based on initial MSG content was obtained since yeast extract acts as a secondary source of substrate. By proper optimization with the simplest medium using L. plantarum, it can produce a high titer of GABA as well as might help various functional foods containing GABA to develop. Graphical abstract: Image 1 Highlights: Synthetic medium for efficient production of GABA was designed. The amounts of yeast extract, glucose, and MSG were optimized for maximum production of GABA. L. plantarum EJ2014 produced 19.8 g/L of GABA, which is the highest. Among the medium components, yeast extract acted also as a secondary substrate.