Assessing the impact of nitrogen supplementation in oats across multiple growth locations and years with targeted phenotyping and high-resolution metabolite profiling approaches. (1st September 2021)

• Record Type: Journal Article
• Authors: Allwood, J. William; Martinez-Martin, Pilar; Xu, Yun; Cowan, Alexander; Pont, Simon; Griffiths, Irene; Sungurtas, Julie; Clarke, Sarah; Goodacre, Royston; Marshall, Athole; Stewart, Derek; Howarth, Catherine
• Is Part Of: Food chemistry. Volume 355(2021)
• Publication Date: 2021-09-01
• Subjects: Metabolomics -- Oats (Avena sativa L.) -- Nitrogen -- Grain quality -- β-glucan -- Proteins and amino acids -- Lipids -- Avenanthramides; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.129585
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: The response to nitrogen of 4 winter oat varieties in three field trials was analysed. A novel high-resolution method was developed to profile metabolite changes. Conditions that enhance yield do not necessarily result in higher nutritional value. Choice of variety is of equally high importance as the nitrogen levels applied. Abstract: Oats ( Avena sativa L.) are a healthy food, being high in dietary fibre (e.g. β-glucans), antioxidants, minerals, and vitamins. Understanding the effect of variety and crop management on nutritional quality is important. The response of four oat varieties to increased nitrogen levels was investigated across multiple locations and years with respect to yield, grain quality and metabolites (assessed via GC- and LC- MS). A novel high-resolution UHPLC-PDA-MS/MS method was developed, providing improved metabolite enrichment, resolution, and identification. The combined phenotyping approach revealed that, amino acid levels were increased by nitrogen supplementation, as were total protein and nitrogen containing lipid levels, whereas health-beneficial avenanthramides were decreased. Although nitrogen addition significantly increased grain yield and β-glucan content, supporting increasing the total nitrogen levels recommended within agricultural guidelines, oat varietal choice as well as negative impacts upon health beneficial secondary metabolites and the environmental burdens associated with nitrogen fertilisation, require further consideration.