A novel subtilin-like lantibiotics subtilin JS-4 produced by Bacillus subtilis JS-4, and its antibacterial mechanism against Listeria monocytogenes. (May 2021)

• Record Type: Journal Article
• Authors: Wei, Zhaohui; Shan, Chengjun; Zhang, Lixia; Ge, Da'e; Wang, Ying; Xia, Xiudong; Liu, Xiaoli; Zhou, Jianzhong
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 142(2021)
• Publication Date: 2021-05
• Subjects: Subtilin JS-4 -- Bacteriocin -- Bacillus subtilis -- Listeria monocytogenes -- Antibacterial mechanism; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2021.110993
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: The foodborne pathogen Listeria monocytogenes is the major cause of listeriosis. We isolated a novel lantibiotic subtilin JS-4 from Bacillus subtilis JS-4 and analyzed its effects against L . monocytogenes . The bacteriocin was extracted with n-butanol, purified by Sephadex LH-20 gel-filtration chromatography and semi-preparative RP-HPLC, and characterized by MALDI-TOF-MS and MALDI-TOF-MS/MS. The molecular mass of subtilin JS-4 was 3346.6 Da, and its amino acid sequence was similar to that of subtilin except for the Ala 15→Val and Leu 24→Ile substitutions. Subtilin JS-4 showed good thermal and pH stability but was inactivated by proteases. It also showed a broad antimicrobial spectrum against Gram-positive bacteria. The antibacterial mechanism of subtilin JS-4 against L. monocytogenes was investigated using confocal laser-scanning microscopy, scanning and transmission electron microscopy, which revealed that subtilin JS-4 increased cell membrane permeability, triggered K + leakage and pore formation, damaged cell membrane integrity, altered cell morphology and intracellular organization. Taken together, subtilin JS-4 is highly effective against foodborne pathogens, and should be considered as an antibacterial preservative in the food industry. Highlights: Subtilin JS-4 was a novel subtilin-like bacteriocin. Subtilin JS-4 killed L. monocytogenes cells by damaging cell membrane integrity and permeability. Subtilin JS-4 showed a broad antimicrobial spectrum against Gram-positive bacteria.