Near-infrared mediated polymer-coated carbon nanodots loaded cisplatin for targeted care management of lung cancer therapy. (December 2020)

• Record Type: Journal Article
• Authors: Fang, Wei; Jin, Ronghui; Mu, Wei
• Is Part Of: Process biochemistry. Volume 99(2020)
• Page Start: 27
• Page End: 35
• Publication Date: 2020-12
• Subjects: TEM Transmission electron microscopy -- DLS Dynamic light scattering -- FBS Fetal bovine serum -- RPMI Roswell Park Memorial Institute Medium -- MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide) -- NIR Near infrared -- FITC Fluorescein isothiocyanate -- DAPI 4′, 6-diamidino-2-phenylindole -- AOEB Acridine orange-Ethidium bromide -- PEG poly ethylene glycol; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.08.009
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: We have effectively synthesized the PEGlyated carbon nanodots loaded cisplatin was fabricated and characterised. It shows excellent in vitro cytotoxicity against lung cancer cells and non-cancerous cell. Further, examined the morphological changes in biochemical staining methods. The apoptosis mechanism was confirmed by flow cytometry analysis and was quantified. Abstract: The Cisplatin and its analogues are scarce for the second leading human health problem, cancer. Therefore, we have effectively engineered the PEGlyated carbon nanodots loaded with cisplatin PEG-CDs@Cis-Pt(IV) for targeted lung cancer therapy. The PEG-CDs@Cis-Pt(IV) confirmed by the electroscopic and spectroscopic methods. The in vitro cytotoxicity of the nanoparticles evaluated two lung cancers (A549 and HEL-299), and one non-cancerous cell line (HUVECs) were used in this study by using MTT assay. The nanoparticles are effectively killing cancer cells without affecting the nano-cancerous cells. Further, dual AO-EB fluorescent staining assay established the programmed cell death through cell morphological changes. Further, flow cytometry confirmed the induction of apoptosis by S phase arrest of the cancer cell cycle by the complexes. The results of our investigations also bear witness to CDs@Cis-Pt(IV)+NIR-NPs promising scope and potency care management for precise cancer therapy beyond platinum drugs.