The effect of ultrasonication on enzymatic hydrolysis of chitin to N-acetyl glucosamine via sequential and simultaneous strategies. (December 2020)

• Record Type: Journal Article
• Authors: Wang, Yingying; Zhang, Alei; Mo, Xiaofang; Zhou, Ning; Yang, Sai; Chen, Kequan; Ouyang, Pingkai
• Is Part Of: Process biochemistry. Volume 99(2020)
• Page Start: 265
• Page End: 269
• Publication Date: 2020-12
• Subjects: Ultrasonication -- Chitin -- GlcNAc -- Chitinase -- Pretreatment; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.09.013
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Ultrasonic treatment lead to chitin to a fluffy and fiber structure with decrease of crystallinity. Ultrasonic treatment does not change the chemical composition of chitin. The concentration of GlcNAc using the simultaneous ultrasonic strategy is 5.00-fold higher than that without treatment. Abstract: In this study, sequential and simultaneous strategies of ultrasonication (ultrasonic bath) were investigated to enhance enzymatic production of N -acetyl glucosamine (GlcNAc) from chitin powder. For the sequential strategy, the ultrasonic caused chitin powder to a visible fleecy structure, and Scanning electron microscopy (SEM) showed the surface of the treated chitin had a fiber-like structure with a diameter of 50 − 200 nm. Moreover, Fourier transform infrared spectroscopy (FTIR), Element analysis (EA), and X-ray diffraction (XRD) revealed that the crystallinity of the chitin decreased with little deacetylation. The simultaneous strategy is a one-pot treatment and enzymatic hydrolysis of chitin. The concentration of GlcNAc was 2.65 g/L for the strategy, which was 1.18- and 5.0-fold higher than the sequential strategy (2.25 g/L) and untreated chitin (0.53 g/L), respectively. In conclusion, this approach provided an efficient and environmentally friendly method for reducing the crystallinity of chitin and enhancing its enzymatic hydrolysis.