Enzymatic hydrolysate of geniposide directly acts as cross-linking agent for enzyme immobilization. (December 2020)

• Record Type: Journal Article
• Authors: Ouyang, Jie; Pu, Shujin; Wang, Juzheng; Deng, Yuefeng; Yang, Chengli; Naseer, Sidra; Li, Dali
• Is Part Of: Process biochemistry. Volume 99(2020)
• Page Start: 187
• Page End: 195
• Publication Date: 2020-12
• Subjects: β-Glucosidase -- Genipin -- Laccase -- Enzyme immobilization; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2020.09.006
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: The chitosan@β-glucosidase was used to hydrolyze natural product geniposide. Enzymatic hydrolysate of geniposide as cross-linking agent directly immobilized laccase. The Nano-SiO2 @laccase has excellent enzymatic properties and great reusability. Abstract: Enzyme immobilization is a routine biotechnology of many industries such as pharmaceutical, chemical and food. Among the different techniques of enzyme immobilization, cross-linking methods are often used. Geniposide is a natural product extracted from gardenia and its hydrolysate genipin is one of green cross-linking agent for enzyme immobilization, but the environmental pollution and cost of the genipin extraction process have become the main obstacle to its wide application. Enzyme β-glucosidase was immobilized on chitosan by self-catalysis and further used to hydrolyze geniposide. The laccase was immobilized on Nano-SiO2 through the hydrolysate of geniposide directly acts as cross-linking agent. The simplification of the extraction steps overcomes the obstacles to the widespread use of genipin. Compared with the free laccase, the Nano-SiO2 @laccase exhibited better pH stability and thermal stability. The Nano-SiO2 @laccase was used to degrade Bisphenol A (BPA) and the biodegradation efficiency of the Nano-SiO2 @laccase was 84.3 % after 10 cycles of reusing.