Eucaloric diets enriched in palm olein, cocoa butter, and soybean oil did not differentially affect liver fat concentration in healthy participants: a 16-week randomized controlled trial. Issue 2 (31st December 2020)

• Record Type: Journal Article
• Authors: Stonehouse, Welma; Sergi, Domenico; Benassi-Evans, Bianca; James-Martin, Genevieve; Johnson, Nathan; Thompson, Campbell H; Abeywardena, Mahinda
• Is Part Of: American journal of clinical nutrition. Volume 113:Issue 2(2021)
• Page Start: 324
• Page End: 337
• Publication Date: 2020-12-31
• Subjects: palm olein -- cocoa butter -- soybean oil -- saturated fatty acids -- polyunsaturated fatty acids -- liver fat -- visceral adipose tissue -- subcutaneous adipose tissue -- fecal fat -- regio-specificity; Diet therapy -- Periodicals; Nutrition -- Periodicals; Dietetics -- Periodicals; 613.205
• DOI: 10.1093/ajcn/nqaa347
• Languages: English
• ISSNs: 0002-9165

Abstract

ABSTRACT: Background: Effects of dietary fat quality on liver fat remain to be elucidated. Inconsistent evidence may be influenced by fatty acid saturation, chain-length, and regio-specificity within triacylglycerol (TAG) molecules. Objectives: We aimed to compare eucaloric diets enriched in palm olein (POo), cocoa butter (COB), and soybean oil (SBO) on liver fat concentration in healthy participants. Secondary outcomes included visceral (VAT) and abdominal subcutaneous (aSCAT) adipose tissue, plus other obesity and cardiometabolic health outcomes. Methods: Eighty-three healthy participants (20–45 y, BMI 18.5–27.5 kg/m 2 ) commenced and 64 completed a 16-wk randomized parallel intervention, preceded by a 2-wk run-in. Participants consumed identical eucaloric background diets differing in test fats [contributing 20% total energy intake (%E)], providing 33%E total fat with the following ratios for PUFAs/SFAs/MUFAs: POo, 4.2/13.5/15%E; SBO, 14.4/8.8/9.4%E; COB, 2.3/19.5/11%E. Liver fat and abdominal adiposity were measured at weeks 0 and 16 using 1 H-magnetic resonance spectroscopy/imaging; all other outcomes were measured at 0, 4, 8, 12, and 16 wk. Results: Fat quality did not affect liver fat concentration, VAT, aSCAT, obesity indexes, blood pressure, liver enzymes, leptin, or fasting glucose. Body fat mass decreased with SBO and COB compared with POo. SBO decreased serum total cholesterol (TC), LDL cholesterol, and TC:HDL cholesterol relative to POo [estimated marginal mean (95% CI) differences: −0.57 (−0.94, −0.20) mmol/L; −0.37 (−0.68, −0.07) mmol/L; and −0.42 (−0.73, −0.11) mmol/L, respectively]. No diet differences were observed on HDL cholesterol, TAG, apoA1, apoB, apoB:apoA1, or fecal free fatty acids (FFAs), except for lower FFA pentadecanoic acid (15:0) with COB than with SBO and POo. Conclusions: In healthy adults, when consumed as part of eucaloric typical Australian diets, 3 different dietary fat sources did not differentially affect liver fat concentration and amounts of adipose tissue. Effects on serum lipids were inconsistent across lipid profiles. The findings must be confirmed in metabolically impaired individuals before recommendations can be made. Abstract : This trial was registered at anzctr.org.au as ACTRN12618000201279.