Thermostable amidase catalyzed production of isonicotinic acid from isonicotinamide. Issue 9 (September 2015)

• Record Type: Journal Article
• Authors: Mehta, Praveen Kumar; Bhatia, Shashi Kant; Bhatia, Ravi Kant; Bhalla, Tek Chand
• Is Part Of: Process biochemistry. Volume 50:Issue 9(2015:Sep.)
• Page Start: 1400
• Page End: 1404
• Publication Date: 2015-09
• Subjects: Geobacillus subterraneus -- Thermostable -- Amidase -- Isonicotinamide -- Isonicotinic acid -- Fed batch; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2015.05.013
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Biotransformation of isonicotinamide to isonicotinic acid at 1 L fed batch scale. Different process parameters were optimized for production of isonicotinic acid. Amidase of G. subterraneus RL-2a showed superior operational thermostability. Isonicotinic acid was produced (61.55 g) at a rate of 1.18 g h −1 g dcw −1 . Abstract: The biotransformation of isonicotinamide was investigated using thermophilic intracellular amidase produced from Geobacillus subterraneus RL-2a. Various process parameters, including amount of biocatalyst, substrate feeding rate, enzyme-to-substrate ratio and operational thermostability were systematically examined with the aim of achieving complete substrate conversion and high productivity. In 1 L fed batch reaction containing 0.1 M isonicotinamide, in 0.2 M potassium phosphate buffer (pH 6.5, 200 rpm) and 8 U ml −1 amidase activity (12.48 mg dcw ml −1 ) of whole cells of G. subterraneus RL-2a (as biocatalyst) resulted in a yield of 0.1 M of isonicotinic acid after 50 min reaction time at 70 °C and a total of 61.55 g isonicotinic acid was produced at a rate of 1.18 g h −1 g −1 dcw respectively. The volumetric productivity was 14.8 g h −1 l −1 .