Monodisperse microsphere-based immobilized metal affinity chromatography approach for preparing Antarctic krill phospholipids followed by HILIC-MS analysis. (15th May 2021)

• Record Type: Journal Article
• Authors: Shen, Qing; Wu, Huanming; Wang, Honghai; Zhao, Qiaoling; Xue, Jing; Ma, Jianfeng; Wang, Haixing
• Is Part Of: Food chemistry. Volume 344(2021)
• Publication Date: 2021-05-15
• Subjects: Monodisperse microsphere -- Immobilized metal affinity chromatography -- Lipidomics -- Krill -- Mass spectrometry; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.128585
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: MM-IMAC was synthesized with Ti 4+ incorporated. Phospholipids in krill were extracted by coordination with the phosphate group. The extract was lipidomics analyzed by HILIC-MS under optimized conditions. MM-Ti 4+ -IMAC-HILIC-MS was reliable and efficient for the lipidomics study of food matrix. Abstract: Phospholipids enriched krill is a functional food beneficial in cardiovascular diseases. Herein, monodisperse microsphere-based immobilized metal affinity chromatographic material (MM-IMAC) was synthesized with Ti 4+ incorporated to enrich phospholipids from krill by coordination with phosphate group. The extract was profiled by hydrophilic interaction chromatography-mass spectrometry (HILIC-MS) with 154 phospholipid molecular species detected. The parameters were loading solvent n -hexane/isopropanol (2:8, v/v), flow rate 0.8 mL·min −1, and eluting volume 1 mL. Besides, eicosapentaenoic and docosahexaenoic acids structured phospholipids were located, such as phosphatidylcholine (PC) 20:5/22:6, phosphatidylinositol (PI) 18:0/20:5, etc. Finally, this method was validated in linearity (R 2 ≥ 0.9953), sensitivity (LOD ≤ 0.53 μg·mL −1 and LOQ ≤ 1.66 μg·mL −1 ), precision (RSDintraday ≤ 4.86% and RSDinterday ≤ 6.25%), and recovery (58–83%). It indicated that the MM-Ti 4+ -IMAC-HILIC-MS was reliable and efficient in specific study of phospholipids in food matrix.