Structure-activity relationship of antioxidant polysaccharides from Salvia miltiorrhiza based on multiple fingerprint profiles and chemometrics analysis. (15th January 2023)

• Record Type: Journal Article
• Authors: Jing, Yongshuai; Zhang, Shilin; Wang, Feifan; Hu, Beibei; Sun, Shiguo; Zhang, Danshen; Zheng, Yuguang; Wu, Lanfang
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 174(2023)
• Publication Date: 2023-01-15
• Subjects: Salvia miltiorrhiza -- Antioxidant polysaccharides -- Fingerprint -- Structure-activity relationship -- Quality assessment; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2023.114473
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: The polysaccharides of Salvia miltiorrhiza (SMP) from 10 different sources were obtained by water extraction and alcohol precipitation, and their physicochemical properties and structures were analyzed. Multidimensional fingerprints based on high-performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC) and proton nuclear magnetic resonance ( 1 H-NMR) were established, and monosaccharide composition (galactose, glucose), molecular weight (SP1 2.07 × 10 6 Da-5.57 × 10 6 Da, SP3 692.20 Da–707.22 Da) and chemical shift (δ2 4.982 ppm, δ4 5.317 ppm) could be used for the quality control of SMP. The antioxidant activity was evaluated, and the spectrum effect relationship was studied. Galactose in the monosaccharide composition and the 2.07 × 10 6 Da-5.57 × 10 6 Da component in molecular weight were found to have a positive correlation effect on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, which can provide a preliminary basis and data reference for the quality control and structure-activity relationship study of SMP. Highlights: Multiple fingerprints were established for Salvia miltiorrhiza polysaccharides (SMP). Chemometrics analysis of SMP was based on multiple fingerprints instead of single. The quality control of SMPs can be well carry out based on multiple fingerprints. The fingerprint-activity relationship of SMP was studied for the first time.