Visual detection of Staphylococcus aureus based on immunomagnetic separation and polymerase spiral reaction. (May 2023)

• Record Type: Journal Article
• Authors: Zhai, Yue; Yu, Haoyan; Liu, Xingxing; Zhang, Mengfan; Han, Rongpeng; Yin, Caihong; Liu, Xingchi; Li, Hui; Li, Juan; Song, Xiuling
• Is Part Of: Food control. Volume 147(2023)
• Publication Date: 2023-05
• Subjects: Staphylococcus aureus -- Phage display technology -- Immunomagnetic separation technology -- Polymerase spiral reaction technology -- Visual detection; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2023.109621
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Staphylococcus aureus ( S. aureus ) is widely distributed in the environment, which can easily lead to food contamination. Thus, it is essential to develop rapid and on-site detection of S. aureus . In the present study, an intuitive colorimetric method based on phage display technology (PDT), immunomagnetic separation (IMS), and polymerase spiral reaction (PSR) was successfully established for S. aureus detection. Peptide-2 (P2, MNVTFTT) with high affinity and specificity for S. aureus was screened by PDT. The formed P2-magnetic beads (MBs) were able to effectively enrich and isolate S. aureus from complex matrices under applied magnetic field. Then a PSR assay was developed for signal amplification and visualization and performed in a thermostatic metal bath using specific primers targeting nuc gene. The results could be observed by naked eyes with a significant color change from purple to blue. The newly developed IMS-PSR assay enabled detection of S. aureus at levels as low as 84 CFU/mL (1.5 pg) in spiked milk sample within 3 h. With all these benefits, it shows promise for rapid on-site visual detection of S. aureus . Highlights: A phage peptide was generated with an amino acid sequence of MNVTFTT. A method was developed for the rapid detection of Staphylococcus aureus. The detection limit reached 84 CFU/mL in a spiked milk sample within 3 h.