Novel polysaccharide and polysaccharide-peptide conjugate from Rosa rugosa Thunb. pseudofruit – Structural characterisation and nutraceutical potential. (30th May 2023)

• Record Type: Journal Article
• Authors: Olech, Marta; Cybulska, Justyna; Nowacka-Jechalke, Natalia; Szpakowska, Nikola; Masłyk, Maciej; Kubiński, Konrad; Martyna, Aleksandra; Zdunek, Artur; Kaczyński, Zbigniew
• Is Part Of: Food chemistry. Volume 409(2023)
• Publication Date: 2023-05-30
• Subjects: Functional food ingredients -- Structural analysis -- Bioactive plant metabolites -- Carbohydrates -- Polysaccharide-protein complex -- Rose hip; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.135264
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Two novel polysaccharides were isolated with high extraction yields from rose hip. Physiochemical characterisation of new macromolecules was provided. One of the macromolecules was found to be a polysaccharide-peptide conjugate. In vitro studies revealed strong α-glucosidase inhibitory activity of the isolated compounds. R. rugosa polysaccharide exhibited considerable antiproliferative potential. Abstract: A novel bioactive polysaccharopeptide (C1) and polysaccharide (C2) with an average molecular weight of 180 kDa and 70 kDa were isolated from R. rugosa pseudofruit. The composition of the macromolecules was established using 1 H NMR, FT-IR, GC–MS, SDS-PAGE coupled with enzymatic cleavage, and proteomic analyses (LC-MS). C1 was found to contain 60.56 ± 1.82 % of sugars and 21.17 ± 0.47 % of uronic acids. Its main neutral monosaccharides were arabinose, rhamnose, galactose, glucose, fucose, and mannose. C1 was found to be a polysaccharopeptide containing pectinesterase-like protein. C2 was composed of 32.85 ± 0.97 % of sugars and 48.77 ± 1.15 % of uronic acids. Its main neutral monosaccharides were galactose, glucose, rhamnose, arabinose, and mannose. A promising nutraceutical value of the polysaccharides was revealed. Assays showed strong α-glucosidase inhibitory activity of both macromolecules and considerable antiradical potential and moderate lipoxygenase inhibitory activity of the crude polysaccharide. Moreover, antiproliferative activity of C2 was observed.