Efficient preparation of dual-emission ratiometric fluorescence sensor system based on aptamer-composite and detection of bis(2-ethylhexyl) phthalate in pork. (1st August 2021)

• Record Type: Journal Article
• Authors: Wang, Yueying; Li, Wenting; Hu, Xuetao; Zhang, Xinai; Huang, Xiaowei; Li, Zhihua; Li, Miaoyun; Zou, Xiaobo; Shi, Jiyong
• Is Part Of: Food chemistry. Volume 352(2021)
• Publication Date: 2021-08-01
• Subjects: Molybdenum disulfide quantum dots -- Cadmium telluride quantum dots -- Aptamer -- Bis (2-ethylhexyl) phthalate -- Ratiometric fluorescence sensor system; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2021.129352
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: MoS2 QDs/CdTe-Apta ratiometric fluorescence sensor system was proposed for DEHP detection in pork. Further validation of the fluorescence quenching mechanism. MoS2 QDs/CdTe-Apta has better detection stability than the single-emission CdTe-Apta. Abstract: A ratiometric fluorescence sensor system is proposed for detecting bis(2-ethylhexyl) phthalate (DEHP) in pork, which is based on aptamer recognition with molybdenum disulfide quantum dots and cadmium telluride quantum dots (MoS2 QDs/CdTe-Apta). Two signals exist in the system, among which the response signal is transmitted by CdTe-Apta. The amide condensation between aptamers and CdTe QDs shortens the distance between CdTe QDs and DEHP, thus quenching the fluorescence of CdTe QDs, possibly through a photoinduced electron transfer mechanism. The MoS2 QDs deliver the self-calibration signal, and the fluorescence of MoS2 QDs remains almost constant when co-existing with DEHP. Linearity (R 2 = 0.9536) was established for the DEHP concentration range 0.005–3.0 mg·L −1, with a limit of detection of 0.21 μg·L −1 . The system was successfully applied in the determination of DEHP in pork. The system has potential for the quantitative determination of DEHP in practical applications.