Characterization of ultrasonically extracted flaxseed polysaccharide gum and assessing its lipid‐lowering potential in a rat model. Issue 1 (17th October 2022)

• Record Type: Journal Article
• Authors: Akhtar, Muhammad Nadeem; Khalil, Anees Ahmed; Bilal, Ahmed; Afzaal, Muhammad; Tufail, Tabussam; Saeed, Rabia; Siddique, Rabia; Nemat, Arash; Manzoor, Muhammad Faisal
• Is Part Of: Food science & nutrition. Volume 11:Issue 1(2023)
• Page Start: 137
• Page End: 147
• Publication Date: 2022-10-17
• Subjects: 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH) -- antioxidant potential -- flaxseed polysaccharide gum (FPG) -- hyperlipidemia; Food industry and trade -- Periodicals; Food -- Periodicals; Nutrition -- Periodicals; 664
• DOI: 10.1002/fsn3.3045
• Languages: English
• ISSNs: 2048-7177

Abstract

Abstract: Flaxseed polysaccharide gum (FPG) was extracted through the ultrasound‐assisted process using water as a solvent with a yield ranging from 8.05 ± 0.32% to 12.23 ± 0.45% by changing different extraction variables. The extracted FPG was analyzed for its functional groups and antioxidant potential. The maximum DPPH (2, 2‐diphenyl‐1‐picrylhydrazyl) free radical scavenging activity (≈100%) of FPG was noted at concentrations beyond ≈10 mg·ml −1 . The maximum inhibition percentage through ABTS (2, 2′‐azino‐bis 3‐ethylbenzothiazoline‐6‐sulfonic acid) (72.4% ± 1.9%) was noted at 40 mg·ml −1, which was observed to be less when compared to DPPH at the same concentration. The total antioxidant potential of the FPG solution at a concentration of 10 mg·ml −1 was equivalent to 461 mg ascorbic acid, which tends to increase with concentration at a much lower scope. The in vivo trial suggested that the least weight gain was noted in experimental groups G2 and Gh2 . A significant reduction in total cholesterol was noticed in G1 (−14.14%) and G2 (−17.72%) and in Gh1 (−22.02%) and Gh2 (−34.68%) after 60 days of the trial compared to the baseline values. The maximum reduction in total triglyceride was observed in Gh2 (−25.06%) and Gh1 (−22.01%) after 60 days of the trial. It was an increasing trend in high‐density lipoprotein cholesterol (HDL‐c) in different experimental groups G2 (10.51%) than G1 (5.35%) and Gh2 (48.96%) and Gh1 (31.11%), respectively, after 60 days of study interval. Reduction of −5.05% and − 9.45% was observed in G1 and G2, while similar results were observed in Gh1 and Gh2 . Conclusively, results suggested a possible protective role of FPG against hyperlipidemia. Abstract : On the basis of current research it was suggested that FPG have anti‐hyperlipidemic potential. Still, the mechanism of actions of FPG as anti‐hyperlipidemic and other therapeutic potential have yet to be explored.