A multiomics integrative analysis of color de-synchronization with softening of 'Hass' avocado fruit: A first insight into a complex physiological disorder. (15th May 2023)

• Record Type: Journal Article
• Authors: Núñez-Lillo, Gerardo; Ponce, Excequel; Arancibia-Guerra, Camila; Carpentier, Sebastien; Carrasco-Pancorbo, Alegría; Olmo-García, Lucía; Chirinos, Rosana; Campos, David; Campos-Vargas, Reinaldo; Meneses, Claudio; Pedreschi, Romina
• Is Part Of: Food chemistry. Volume 408(2023)
• Publication Date: 2023-05-15
• Subjects: Transcriptomics -- Proteomics -- Metabolomics -- MixOmics -- Abscisic acid -- Anthocyanins; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.135215
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Integration of omics datasets and network analysis was carried out. Eight transcription factors (TF) associated with differentially regulated genes between RA and CA. Twelve TF associated with avocado fruit color desynchronization control. Higher contents of flavonoids, ABA and brassinosteroids associated with synchronized fruit. Color desynchronization associated with higher JA, SA and auxins levels. Abstract: Exocarp color de-synchronization with softening of 'Hass' avocado is a relevant recurrent problem for the avocado supply chain. This study aimed to unravel the mechanisms driving this de-synchronization integrating omics datasets from avocado exocarp of different storage conditions and color phenotypes. In addition, we propose potential biomarkers to predict color synchronized/de-synchronized fruit. Integration of transcriptomics, proteomics and metabolomics and network analysis revealed eight transcription factors associated with differentially regulated genes between regular air (RA) and controlled atmosphere (CA) and twelve transcription factors related to avocado fruit color de-synchronization control in ready-to-eat stage. CA was positively correlated to auxins, ethylene, cytokinins and brassinosteroids-related genes, while RA was characterized by enrichment of cell wall remodeling and abscisic acid content associated genes. At ready-to-eat higher contents of flavonoids, abscisic acid and brassinosteroids were associated with color-softening synchronized avocados. In contrast, de-synchronized fruit revealed increases of jasmonic acid, salicylic acid and auxin levels.