Fabrication of immobilized lipases for efficient preparation of 1, 3-dioleoyl-2-palmitoylglycerol. (15th May 2023)

• Record Type: Journal Article
• Authors: He, Lihong; Zeng, Can; Wei, Lingfeng; Xu, Li; Song, Fenglin; Huang, Jianrong; Zhong, Nanjing
• Is Part Of: Food chemistry. Volume 408(2023)
• Publication Date: 2023-05-15
• Subjects: AOL Aspergillus oryzae lipase -- AR Analytical Reagent -- BCL lipase from Burkholderia cepacia -- CALA Candida antarctica lipase A -- CALB Candida antarctica lipase B -- CPs coordination polymers -- CRL Candida rugosa lipase -- DAG diacylglycerols -- ECN equivalent carbon number -- EDS energy dispersive spectrometer -- ELSD evaporative light scattering detector -- FAMEs fatty acid methyl esters -- FFAs free fatty acids -- FID flame ionization detector -- GC gas chromatography -- GMP guanosine 5′-monophosphate -- HMF human milk fat -- LU Lecitase® Ultra -- MAG monoacylglycerols -- OOO triolein -- OPL 1-oleoyl-2-palmitoyl-3-linoleoylglycerol -- OPO 1, 3-dioleoyl-2-palmitoyl-glycerol -- PPL Porcine Pancreas lipase -- PPP glycerol tripalmitate -- RML Rhizomucor miehei lipase -- ROL Rhizopus oryzae lipase -- SEM scanning electron microscopy -- TAG triacylglycerols -- TGA thermogravimetric analysis -- TLC thin layer chromatography -- TLL Thermomyces lanuginosa lipase -- XRD X-ray diffraction; 1, 3-dioleoyl-2-palmitoyl-glycerol -- Acidolysis -- Immobilization -- Lipase -- Orthogonal; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.135236
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: TLL@R-SBA-15 samples gave PPP conversion from 97.70 to 99.07% and OPO content from 59.52 to 73.15% TLL@C18 H37 -SBA-15 exhibited better acidolysis performance at 50 °C than that from 60 to 80 °C. TLL@C18 H37 -SBA-15 gave OPO content 73.15 % and sn -2 palmitic acid content 90.09 % 81.04% of the initial activity (based on OPO content) was retained after 5 cycles of reuse. Abstract: This study aims to fabricate immobilized lipases for efficient preparation of 1, 3-dioleoyl-2-palmitoyl-glycerol (OPO) through acidolysis of glycerol tripalmitate (PPP). Twelve (three types) supports and five lipases were studied carefully. Among them, the immobilized Thermomyces lanuginosa lipase (TLL) samples exhibited overall better performance than that of other immobilized lipases. Particularly, organic groups functionalized SBA-15 (R-SBA-15) supported TLL (TLL@R-SBA-15) samples gave PPP conversion from 97.70 to 99.00 % and OPO content from 59.52 to 64.73 %. After optimization, PPP conversion up to 99.07 %, OPO content 73.15 % and sn -2 palmitic acid content 90.09 % were obtained with TLL@C18 H37 -SBA-15 as catalyst. Moreover, TLL@C18 H37 -SBA-15 exhibited better acidolysis performance from 50 °C than that from 60 to 80 °C, which helped inhibit acyl migration. In addition, after 5 cycles of reuse, TLL@C18 H37 -SBA-15 retained 81.04 % (based on OPO content) and 98.88 % (based on sn- 2 palmitic acid content) of its initial activity, indicating it had an attractive prospect in future applications.