The eisosomes contribute to acid tolerance of yeast by maintaining cell membrane integrity. (April 2023)

• Record Type: Journal Article
• Authors: Li, Chao; Lu, Jun; Yan, Xue-Jiao; Li, Chang-Wen; Lin, Liang-Cai; Xiao, Dong-Guang; Zhang, Cui-Ying
• Is Part Of: Food microbiology. Volume 110(2023)
• Publication Date: 2023-04
• Subjects: Eisosome -- Acid tolerance -- Lipidomics -- Yeast; WT wild type -- FI fluorescence intensity -- CLSM Confocal Laser Scanning Microscopy -- TEM Transmission electron microscopy -- LC-MS Liquid Chromatography-Mass Spectrometry -- (MTBE) methyl-tert-butyl ether -- ER endoplasmic reticulum -- VAPs VAMP-associated proteins -- PC phosphatidylcholines -- PI phosphatidylinositols -- PE phosphatidylethanolamines -- PS phosphatidylserines -- PG phosphatidylglycerols -- LPE lyso-PE -- IPC inositolphosphoryl ceramide -- OPLS-DA Orthogonal Partial Least-Squares Discrimination Analysis; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2022.104157
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Microbes have evolved multiple mechanisms to resist environmental stresses, which are regulated in complex and delicate ways. Though the role of cell membranes in acid resistance from the perspective of physicochemical properties and membrane proteins has been deeply studied, the function of eisosomes is still in its infancy. In this study, we firstly reported the dynamic changes of eisosomes under acid stress and the decreased acid tolerance of yeasts caused by eisosome disruption. Physiological indicators and non-targeted lipid profiling revealed that eisosome disruption caused changes in multiple lipids and imbalances in lipid homeostasis, which are responsible for membrane integrity damage. Thus the increased infiltration of carboxylic acids and the raised ROS levels were detected in strains with disrupted eisosome assembly, resulting in decreased cellular tolerance. The results here provide novel insights into the acid-resistant mechanism of yeasts from the perspective of the cell membrane subdomain, which has practical impacts on green biological manufacturing and food preservation. Graphical abstract: Image 1 Highlights: Eisosomes are indispensable for yeasts to resist acetic and lactic acid stress. Eisosome are responsible for maintaining cell membrane integrity. Disruption of eisosomes blocked the elongation and desaturation of lipid acyl chain.