Soluble extracellular polymeric substance (SEPS) of histo-blood group antigen (HBGA) expressing bacterium Sphingobacterium sp. SC015 influences the survival and persistence of norovirus on lettuce. (February 2023)

• Record Type: Journal Article
• Authors: Liao, Ningbo; Tang, Mengxuan; Chen, Lili; Tian, Peng; Wang, Dapeng; Cheng, Dongqing; Wu, Guoping
• Is Part Of: Food microbiology. Volume 109(2023)
• Publication Date: 2023-02
• Subjects: Norovirus -- Bacteria -- Lettuce -- Soluble extracellular polymeric substance (SEPS) -- Histo-blood group antigen (HBGA) -- Thermal -- Hypochlorite -- Ultraviolet; HBGA Histo-blood group antigen -- SEPS Soluble extracellular polymeric substance -- SC015 Sphingobacterium sp. SC015 -- SEPS-SC015 Soluble extracellular polymeric substances from bacteria SC015 -- SEPS-EC Soluble extracellular polymeric substances from E. coli K-12 -- HuNoV Human norovirus -- TuV Tulane virus -- MNV murine norovirus -- ATCC American Type Culture Collection -- PFU Plaque forming units -- PS penicillin/streptomycin -- DMEM Dulbecco's modified Eagle medium -- FBS fetal bovine serum -- PGM porcine gastric mucin -- PBS phosphate-buffered saline; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2022.104126
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: Foodborne norovirus (NoV) outbreaks linked to leafy greens are common due to a lack of efficient strategies to prevent NoV spread from contaminated surfaces. We previously found that Sphingobacterium sp. SC015 in lettuce phyllosphere expresses histo-blood group antigen (HBGA)-like substances in soluble extracellular polymeric substances (SEPS) that contribute to NoV adherence on lettuce. Here, we extracted SEPS from bacterium SC015 (SEPS-SC015), analyzed their chemical composition, and examined their roles in the survival and protection of NoV and surrogates [murine norovirus (MNV-1) and Tulane virus (TuV)] on lettuce. Presence of SEPS-SC015 significantly increased survival and persistence of human NoV (HuNoV), MNV-1, and TuV at days 7 and 14, compared with virus alone. HuNoV, TuV, and MNV-1 seeded with SEPS-SC015 were more resistant to heat (70 °C, 2 min) than these viruses alone. SEPS-SC015 also increased viral resistance to sodium hypochlorite inactivation by treatment with 30 and 300 ppm bleach at 26 °C for 10 min. However, SEPS-SC015 was not effective at protecting these viruses under UV inactivation. Binding of TuV to SC015 bacteria and SEPS-SC015, visualized using transmission electron microscopy, suggests that protection might be related to direct interaction between SEPS-SC015 and viral particles. This study provides important insights that will help inform strategies to improve food safety. Graphical abstract: Image 1 Highlights: The Sphingobacterium sp . SC015 in lettuce phyllosphere expresses histo-blood group antigen (HBGA) substances. The HBGA substances are mainly distributed in the soluble extracellular polymeric substance (SEPS-SC015). SEPS-SC015 increases the survival and persistence of HuNoV, MNV-1, and TuV. SEPS-SC015 confers protective effects on HuNoV, MNV-1 and TuV against heat and chemical inactivation. The protection might be related to affinity interactions between SEPS-SC015 and viral particle.