Comprehensive metabolomic profiling of nutrients in fish and shrimp. (1st May 2023)

• Record Type: Journal Article
• Authors: Shao, Chang; Su, Yang; Meng, Duanyue; Li, Yi; Dong, Yuanyuan; Hao, Haiping; Ye, Hui
• Is Part Of: Food chemistry. Volume 407(2023)
• Publication Date: 2023-05-01
• Subjects: Aquatic products -- Shrimp -- Fish -- Nontargeted metabolomics -- Nutrients -- Anti-inflammation; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.135037
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Metabolomics was employed to identify the nutrients in fish and shrimp species. Beef was analyzed to compare with the nutrients in the examined aquatic animals. 71 metabolites were found differentially expressed among fish, shrimp and beef. Functions of the rich metabolites in fish and shrimp were discussed. Selected metabolites rich in shrimp showed anti-inflammatory activity in vitro . Abstract: Although fish and shrimp are commonly consumed in daily life and recognized as nutrient-rich species, global profiling of the endogenous nutrients in these species is lacking. Here, we optimized the sample preparation and data acquisition methods of metabolomics to comprehensively characterize the nutrients in selected fish and shrimp species and compared them with those in beef, leading to the identification of 71 differentially expressed metabolites. Of these, docosahexaenoic acid, taurine, choline and (lyso)phosphatidylcholines were found to be abundant in the examined fish species, while several nonessential amino acids were rich in the analyzed shrimp samples. Subsequently, the biological functions of the metabolites rich in shrimp were queried. Intriguingly, the examined nutrients exemplified by proline can significantly mitigate the lipopolysaccharide (LPS)-stimulated inflammatory responses in BV2 cells and RAW264.7 cells. Collectively, our findings imply that the dietary intake of certain fish and shrimp species may benefit human health through alleviating inflammatory responses.