Phillygenin from Forsythia suspensa leaves exhibits analgesic potential and anti‐inflammatory activity in carrageenan‐induced paw edema in mice. Issue 12 (6th October 2022)

• Record Type: Journal Article
• Authors: Guo, Jing; Tang, Jian‐Kai; Wang, Bai‐Fang; Yan, Wen‐Rui; Li, Ting; Guo, Xue‐Jian; Zhang, Lei; Wang, Tao; Sun, Qian‐Yun; Zhang, Li‐Wei
• Is Part Of: Journal of food biochemistry. Volume 46:Issue 12(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-10-06
• Subjects: anti‐inflammation -- fermentation -- Forsythia suspensa leaves -- inflammatory mediators -- nuclear factor‐kappa B (NF‐κB) -- pain; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Biochemistry -- Periodicals; 664.024
• DOI: 10.1111/jfbc.14460
• Languages: English
• ISSNs: 0145-8884

Abstract

Abstract: Forsythia suspensa (Thunb.) Vahl (Oleaceae) leaves are valuable sources of phillygenin. This study aimed to isolate phillygenin from F. suspensa leaves and examine its analgesic and anti‐inflammatory effects. Phillygenin was successfully extracted and isolated from F. suspensa leaves after fermentation. Phillygenin significantly reduced the number of writhing induced by acetic acid, prolonged the latency period in the hot plate test, and inhibited the xylene‐induced ear edema and carrageenan‐induced paw edema in mice. IL‐6, TNF‐α, IL‐1β, NO, and PGE2 levels in the carrageenan‐induced paw edema were notably reduced after pretreatment with phillygenin. Phillygenin significantly decreased the iNOS and COX‐2 protein expressions and the IκB‐α and NF‐κB p65 phosphorylation. This study demonstrated that phillygenin is a potential therapeutic candidate for managing pain and inflammation‐mediated disorders. The study contributes to the comprehensive development and utilization of F. suspensa leaves for economic and health care. Practical applications: Phillygenin is one of the major active ingredients in Forsythia suspensa . But the content of phillygenin in F. suspensa is very low which limits its application. Phillygenin has potential pharmacological activity and anti‐inflammatory properties. However, the potential effects of phillygenin on analgesic activity have not been clarified. Furthermore, the data on its anti‐inflammatory activity in vivo are relatively limited. This study evaluated the analgesic activity for the first time and the acute anti‐inflammatory effect of phillygenin from F. suspensa leaves by fermentation, which indicated phillygenin is a potential therapeutic candidate for managing pain and inflammation‐mediated disorders. Abstract : Forsythia suspensa (Thunb.) Vahl (Oleaceae) leaves are valuable sources of phillygenin. Phillygenin was successfully extracted and isolated from F. suspensa leaves after fermentation and reduced the number of writhing induced by acetic acid, prolonged the latency period in the hot plate test, inhibited the xylene‐induced ear edema and carrageenan‐induced paw edema, decreased inflammatory mediators production, and regulated iNOS and COX‐2 and NF‐κB signal proteins expressions. Our study suggest that phillygenin may contribute to managing pain and inflammation‐mediated disorders.