Developing an ultrasensitive immunochromatographic assay for authentication of an emergent fraud aminopyrine in herbal tea. (16th April 2023)

• Record Type: Journal Article
• Authors: Fang, Yalin; Wang, Zian; Quan, Qiqi; Li, Zhaodong; Pan, Kangliang; Lei, Yi; Yao, Xiaojun; Li, Xiangmei; Shen, Xing; Koidis, Anastasios; Lei, Hongtao
• Is Part Of: Food chemistry. Volume 406(2023)
• Publication Date: 2023-04-16
• Subjects: AMI aminopyrine -- AuNPs-ICA Gold nanoparticles based immunochromatographic assay -- BSA bovine serum albumin -- C line control line -- CV the coefficient of variation -- DIM 1, 5-dimethyl-4-(methylamino)-2-phenyl-1, 2-dihydro-3H-pyrazol-3-one -- DMF N, N-dimethylformamide -- EDC 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride -- HPLC high performance liquid chromatography -- IC50 half inhibitory concentration -- icELISA indirect competitive enzyme-linked immunosorbent assay -- IgG Immunoglobulin G -- LC-MS/MS liquid chromatography-tandem mass spectrometry -- LF lactoferrin -- LOD the limit of detection -- LOQ the limit of quantification -- NHS N-hydroxysuccinimide -- OVA ovalbumin -- PVC polyvinyl chloride -- PVP polyvinyl pyrrolidone -- SD the standard deviation -- TEM transmission electron microscope -- TLC thin layer chromatography -- T line test line -- UPLC-MS/MS ultrahigh performance liquid chromatography-tandem mass spectrometry; Aminopyrine -- Herbal teas -- Antibody -- Immunochromatographic assay; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.135065
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Rational hapten design strategy for aminopyrine was provided. A specific antibody with IC50 of 3.00 ng/mL was obtained for the first time. Immunochromatographic assay for aminopyrine in herbal tea was proposed. Abstract: Aminopyrine is a nonsteroidal anti-inflammatory drug only for medical purposes, however, it has been illegally added in traditional Chinese herbal teas for fraud activity recently. In this study, a specific antibody against aminopyrine with IC50 of 3.00 ng/mL was obtained for the first time by a rational hapten design. Furthermore, an ultrasensitive gold nanoparticles immunochromatographic assay (AuNPs-ICA) for determination of aminopyrine based on a portable reader was firstly developed, with cut-off value of 100.00 ng/mL, limit of detection (LOD) of 4.80 ng/mL and limit of quantification (LOQ) of 5.71 ng/mL for herbal tea, respectively. The recovery rates ranged from 93.21 % to 105.61 %, with inter-assay coefficient of variation (CV) from 1.08 % to 3.82 %. Additionally, 24 blind samples were examined simultaneously by AuNPs-ICA and LC-MS/MS, demonstrating a good consistency for each other. The proposed AuNPs-ICA is an ultrasensitive and reliable tool for on-site surveillance screening of fraud additives in herbal tea.