Alkaline phosphatase triggered ratiometric fluorescence immunoassay for detection of zearalenone. (April 2023)

• Record Type: Journal Article
• Authors: Wu, Shixiang; Zhang, Xingping; Chen, Wang; Zhang, Guohao; Zhang, Qian; Yang, Hualin; Zhou, Yu
• Is Part Of: Food control. Volume 146(2023)
• Publication Date: 2023-04
• Subjects: Alkaline phosphatase -- Zearalenone -- Ratiometric fluorescence -- Immunoassay; ZEN zearalenone -- ALP alkaline phosphatase -- OPD o-phenylenediamine -- DAP 2, 3-diaminophenazine -- ppi pyrophosphate -- pi orthophosphates -- PTA-NH2 2-aminoterephthalic acid -- IFE inner filter effect; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2022.109541
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Inspired by the super-binding capacity of pyrophosphate (ppi) and copper ion (Cu 2+ ), an alkaline phosphatase (ALP) triggered ratiometric fluorescence immunoassay was established for detection of zearalenone (ZEN). In the assay, ALP-antibody acted as a bridge connecting ZEN concentration and ratiometric fluorescence signals. Due to the super-binding ability of ppi and Cu 2+, Cu 2+ was immobilized to form ppi-Cu 2+ complex and lost its oxidation activity. The presence of ALP can hydrolyze ppi and release Cu 2+ which can oxidize OPD to DAP. Since the excitation wavelength of DAP coincided with the emission wavelength of 2-aminoterephthalic acid (PTA-NH2 ), a ratiometric fluorescence immunoassay can be developed. The limit of detection (LOD) was 0.014 ng/mL and the linear detection range was 3.51–17.78 ng/mL. The ratiometric fluorescence immunoassay had high accuracy and reliability (recoveries, 101.10–107.20%; coefficient of variation (CV), 2.15–7.65%). This method provided new ideas for efficient and accurate detection of ZEN. Highlights: A ppi/Cu 2+ /OPD/PTA-NH2 -based ratiometric fluorescence immunoassay for zearalenone was developed. In comparison with other immunnoassays, this immunnoassay shows better sensitivity. There was no need to prepare any nanomaterials in advance.