A biosensor based on oriented immobilization of an engineered l-glutamate oxidase on a screen-printed microchip for detection of l-glutamate in fermentation processes. (30th March 2023)

• Record Type: Journal Article
• Authors: Yang, Lu; Bai, Rongrong; Xie, Bin; Zhuang, Nisha; Lv, Ziyao; Chen, Minjiao; Dong, Weiliang; Zhou, Jie; Jiang, Min
• Is Part Of: Food chemistry. Volume 405:Part A(2023)
• Publication Date: 2023-03-30
• Subjects: l-Glutamate biosensor -- l-Glutamate fermentation -- Oriented immobilization -- Chitin-binding domain -- Prussian blue nanocubes; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134792
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: We designed a novel-oriented immobilization strategy for enzyme-based biosensor. This oriented immobilization increased sensitivity and stability of the biosensor. The proposed biosensor displayed a high sensitivity and strong anti-interference. The biosensor performed the quantification of l -glutamate in fermentation without pretreatment. Abstract: This paper describes an amperometric biosensor utilizing an engineered l -glutamate oxidase for glutamate monitoring in microbial fermentation processes. We designed a general immobilization strategy that utilized a chitin-binding domain (ChBD-tag) as a biotether to further immobilize l -glutamate oxidase (GLOX) in an oriented manner on a screen-printed Prussian blue nanocube microchip (PB/SPC) with the biopolymer chitosan. The improved l -glutamate biosensor exhibited an enhanced sensitivity of 53.4 µA L mmol −1 cm −2 and a linear range from 25 µmol/L to 300 µmol/L with a detection limit of 9 µmol/L, and retained 95 % of its initial activity after two weeks of usage. In addition, the as-prepared biosensor was applied for real-time monitoring of food ingredient l -glutamate concentration during the fermentation process, which was in good agreement with that of high-performance liquid chromatography (HPLC). Above all, the l -glutamate biosensor prepared by this method had high analytical performance, and could fully realize real-time and high-efficiency monitoring in glutamate fermentation.