Determination of four aflatoxins on dark tea infusions and aflatoxin transfers evaluation during tea brewing. (30th March 2023)

• Record Type: Journal Article
• Authors: Ye, Ting; Yan, Hangbin; Wang, Xu; Zhao, Weifan; Tao, Fang; Zhou, Yu
• Is Part Of: Food chemistry. Volume 405:Part B(2023)
• Publication Date: 2023-03-30
• Subjects: Dark tea -- Tea infusion -- Aflatoxin -- Method validation -- Tea brewing -- Transfer rate; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134969
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: A QuEChERS method for aflatoxins detection in tea infusion. Dark tea matrix showed strong adsorbability to aflatoxin. Only 2.94% AFB1 was released from the stored fortified dark tea by tea brewing. Abstract: In daily tea drinking, little is known on aflatoxin transfers from tea-leaf to infusion during brewing and the actual intake. A verified aflatoxins analytical method on tea infusion is significant to the exposure assessment. Here, an optimal method 3 (M3) was screened and validated on four aflatoxins (B1, B2, G1 and G2 ) simultaneous determination. Recoveries of AFG1 and AFB1 were 87.37 %±1.99 %-102.03 %±8.62 %, and AFG2 and AFB2 were 83.99 %±8.65 %-100.14 %±1.69 %. The correlation coefficient on the fortified samples determination was > 0.99 for each aflatoxin. In accuracy and precision validation, aflatoxins recoveries in high and low fortified samples were 85.94 %-103.83 %, and RSDs were 2.20 %-9.15 %. Method applicability test showed that the M3 was qualified for six different dark tea types. Extraction rate of 30-days stored AFB1 fortified tea-leaf by acetonitrile–water (84:16, v/v) was < 50 %; meanwhile, only 2.94 % spiked AFB1 was released by ten times tea brewing which indicated that Puerh tea have strong adsorbability to aflatoxin.