A sensitive sensing system based on fluorescence dipeptide nanoparticles for sulfadimethoxine determination. (30th March 2023)

• Record Type: Journal Article
• Authors: Yan, Rongfang; Wen, Zhenhua; Hu, Xuelian; Wang, Wenxiu; Meng, He; Song, Yang; Wang, Shuo; Tang, Yiwei
• Is Part Of: Food chemistry. Volume 405:Part B(2023)
• Publication Date: 2023-03-30
• Subjects: Dipeptide nanoparticles -- Aptamer -- Fluorescence analysis -- Sulfadimethoxine; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.134963
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: TPNPs was conjugated successfully with the SDM aptamer using EDC/NHS-mediated coupling. Tris-HCl buffer was the best solvent based on a strongest fluorescent signal subsequently. Fluorescence was linear in the 0–200 ng/mL sulfadimethoxine. LOD and recoveries were 1.47 ng/mL and 92.88 %-114.15 %, respectively. A new simpler and accurate method was developed (RSD < 5 %). Abstract: Various types of fluorescent nanoparticles have been proposed for the detection of veterinary drug residues in food. However, structure-induced fluorescence nanoparticles with biodegradability and the capacity to conjugate with molecular recognition elements are lacking. Here, a biodegradable tryptophan-phenylalanine dipeptide-based nanomaterial was assembled and modified with a sulfadimethoxine aptamer (TPNPs@aptamer). Then, a novel and efficient fluorescence sensing strategy, based on TPNPs@aptamer and BHQ1-labeled single-stranded DNA (BHQ1@cDNA), was developed for sulfadimethoxine determination. Under optimal conditions, the fluorescence was linear in the range of 0–200 ng/mL sulfadimethoxine with a detection limit of 1.47 ng/mL (S/N = 3). Satisfactory recoveries of standard additions were found between 92.88 % and 114.15 % for water and milk samples with a relative standard deviation less than 5.0 %, suggesting that the proposed fluorescence sensing strategy can be applied reliably in sulfadimethoxine analysis of liquid foods.