Piperine, a component of black pepper, decreases eugenol‐induced cAMP and calcium levels in non‐chemosensory 3T3‐L1 cells. Issue 1 (27th November 2014)

• Record Type: Journal Article
• Authors: Yoon, Yeo Cho; Kim, Sung-Hee; Kim, Min Jung; Yang, Hye Jeong; Rhyu, Mee-Ra; Park, Jae-Ho
• Is Part Of: FEBS open bio. Volume 5:Issue 1(2015)
• Page Start: 20
• Page End: 25
• Publication Date: 2014-11-27
• Subjects: cAMP -- Black pepper -- Olfactory -- Piper nigrum -- Piperine -- 3T3-L1; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.fob.2014.11.008
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : This study investigated the effects of an ethanol extract of black pepper and its constituent, piperine, on odorant‐induced signal transduction in non‐chemosensory cells. An ethanol extract of black pepper decreased eugenol‐induced cAMP and calcium levels in preadipocyte 3T3‐L1 cells with no toxicity. Phosphorylation of CREB (cAMP response element‐binding protein) was down‐regulated by the black pepper extract. The concentration (133.8 mg/g) and retention time (5.5 min) of piperine in the ethanol extract were quantified using UPLC–MS/MS. Pretreatment with piperine decreased eugenol‐induced cAMP and calcium levels in 3T3‐L1 cells. Piperine also decreased the phosphorylation of CREB, which is up‐regulated by eugenol. These results suggest that piperine inhibits the eugenol‐induced signal transduction pathway through modulation of cAMP and calcium levels and phosphorylation of CREB in non‐chemosensory cells. Abstract : An ethanol extract of black pepper inhibits eugenol‐induced signal transduction. The extract reduces eugenol‐induced cAMP and Ca 2+ levels, and phosphorylation of CREB. Piperine inhibits eugenol‐induced signal transduction. Piperine reduces eugenol‐induced cAMP and Ca 2+ levels, and phosphorylation of CREB.